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| Product Name | Sulforhodamine B (SRB) Cytotoxicity Assay Kit |
| Catalog No. | CATR-HMM-0049 |
| Description | A colorimetric assay kit based on the sulforhodamine B protein staining method for quantifying cell density in monolayer cultures. The SRB dye binds stoichiometrically to protein basic amino acid residues under mildly acidic conditions and can be extracted under basic conditions for absorbance measurement. This protein-staining approach provides a stable endpoint that is independent of cellular metabolic state, making it suitable for drug sensitivity testing on cell lines with varying metabolic profiles. |
| Intended Use | Determination of cell density in adherent cultures by protein content staining for cytotoxicity and proliferation studies. |
| Principle / Technology | Sulforhodamine B dye binding to cellular proteins followed by extraction and colorimetric quantification |
| Detection Method | Colorimetric (absorbance at 510–565 nm) |
| Sample Type | Adherent mammalian cell lines in 96-well plate format |
| Performance Range / Specifications | Linear correlation between cell number and absorbance: 500–100,000 cells per well (cell line dependent) |
| Sensitivity / LOD | Detection of approximately 200 cells above background |
| Specificity | Binds cellular proteins non-discriminately; signal proportional to total cellular protein content rather than metabolic activity |
| Reaction Conditions / Protocol | Fix cells with 10% TCA 1 hr at 4 °C; stain with 0.4% SRB 30 min at room temperature; wash 4× with 1% acetic acid; solubilize dye with 10 mM Tris base; read at 540 nm |
| Components / Formulation | Sulforhodamine B powder, trichloroacetic acid (TCA) fixative solution (50% w/v), acetic acid wash solution (1%), Tris base extraction solution (10 mM, pH 10.5) |
| Storage Conditions | Powder: room temperature desiccated; solutions: room temperature |
| Shelf Life | 36 months for SRB powder |
| Package Specifications | 1000 tests per kit (96-well plate format) |
| Product Form | SRB powder; liquid solutions |
| Quality Control | SRB dye purity ≥95% by spectrophotometric analysis; TCA fixation efficacy confirmed with HeLa cell standard |
| Key Features | TCA-fixed plates can be stored for months before staining; independence from metabolic rate avoids drug interference artifacts |
| Purity | Reagent grade; dye purity ≥95% by HPLC; sterile where applicable |
| Concentration | As specified on product label; working concentrations optimized per protocol |
| Activity / Unit Definition | Signal-to-noise ratio and linearity verified on reference cell lines per lot |
| Molecular Weight | Varies by dye or reagent component as specified |
| Source / Origin | Synthetic dyes and tetrazolium compounds; recombinant enzymes where applicable |
| pH Range / Optimal pH | pH 7.2–7.4 for cell-based assays |
| Shipping Conditions | Cold packs; protect light-sensitive reagents from light |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 |
| Compatibility | Compatible with adherent and suspension mammalian cell culture in 96-well and 384-well formats |
| Recommended Buffer System | PBS or phenol red-free complete medium as specified in protocol |
| Application Notes / Precautions | Optimize cell seeding density; include appropriate vehicle controls; avoid phenol red interference with absorbance-based assays |
| Batch-to-Batch Consistency | Signal linearity R² ≥0.99 with reference cell number standard curve per lot |
For research use only, not for clinical use.
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