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| Product Name | ATP Viability Luminescence Assay System |
| Catalog No. | CATR-HMM-0038 |
| Description | A bioluminescence-based ATP detection reagent that provides a rapid and ultra-sensitive method for quantifying viable cells. The assay utilizes recombinant firefly luciferase to generate light proportional to intracellular ATP concentration, which correlates directly with the number of metabolically active cells. |
| Intended Use | High-sensitivity cell viability and proliferation measurement in low-cell-number applications such as stem cell culture, primary cell analysis, drug potency testing, and high-throughput screening. |
| Principle / Technology | ATP released from lysed cells reacts with luciferin and oxygen in the presence of firefly luciferase and Mg2+ to produce oxyluciferin, AMP, CO2, and light (560 nm); luminescence intensity is proportional to ATP concentration under saturating substrate conditions. |
| Detection Method | Bioluminescence measured in relative light units (RLU) using a luminometer or multi-mode plate reader with luminescence detection capability |
| Sample Type | Mammalian cells, insect cells, primary cells, stem cells, bacteria; adherent or suspension cultures in 96-well, 384-well, or 1536-well microplates |
| Performance Range / Specifications | Linear detection range: 10–100,000 cells per well for most mammalian cells; luminescence signal half-life >2 hours |
| Sensitivity / LOD | Detection of fewer than 10 viable cells per well for most transformed cell lines |
| Specificity | Luciferase-luciferin reaction is highly specific for ATP; ADP, AMP, and other nucleotide phosphates produce negligible signal; luminescence background from spent medium is typically very low |
| Reaction Conditions / Protocol | Equilibrate cell plate and reagent to room temperature; add equal volume of reagent to culture medium (e.g., 100 μL reagent to 100 μL medium); mix for 2 minutes on orbital shaker to ensure cell lysis; equilibrate 10 minutes for signal stabilization; read luminescence |
| Components / Formulation | Lyophilized luciferin substrate and recombinant luciferase with buffer salts and stabilizers; reconstitution buffer included; supplied as single-component reagent after reconstitution |
| Storage Conditions | Lyophilized: -20°C protected from light; reconstituted reagent: 2–8°C for 1 week or -20°C for up to 3 months (single freeze-thaw recommended) |
| Shelf Life | 12 months for lyophilized reagent |
| Package Specifications | 10 mL (100 assays in 96-well format), 100 mL (1,000 assays), 10 × 100 mL bulk format |
| Product Form | Lyophilized reagent requiring reconstitution |
| Quality Control | Each lot standardized against known ATP concentrations (0.01 nM–1 μM range); linearity confirmed (R2 ≥0.99); luciferase activity verified to exceed 1 × 10^8 RLU/μL/min |
| Key Features | Homogeneous add-mix-read format; signal half-life >2 hours enabling batch processing; linear response across four orders of magnitude of cell number; compatible with automated liquid handling systems |
For research use only, not for clinical use.
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