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| Product Name | Trypan Blue Exclusion Staining Solution |
| Catalog No. | CATR-HMM-0040 |
| Description | A ready-to-use 0.4% Trypan Blue solution for the classical dye-exclusion method of cell counting and viability assessment. The negatively charged diazo dye is actively excluded by intact cell membranes of viable cells, while non-viable cells with compromised membranes absorb the dye and appear distinctly blue under bright-field microscopy. |
| Intended Use | Routine cell counting and viability monitoring in cell culture laboratories, bioprocessing facilities, and research settings where a rapid, cost-effective viability assessment is needed prior to further experiments. |
| Principle / Technology | Trypan Blue (molecular weight 960.8 Da) carries a net negative charge and is excluded from viable cells with functional membrane integrity; cells with damaged membranes permit dye entry, binding to intracellular proteins and rendering dead cells dark blue under phase-contrast or bright-field microscopy. |
| Detection Method | Bright-field or phase-contrast microscopy with hemocytometer or automated cell counter |
| Sample Type | Single-cell suspensions from mammalian cell culture (adherent or suspension lines), primary cell isolates, dissociated tissue cells, peripheral blood mononuclear cells |
| Performance Range / Specifications | Effective for cell concentrations of 1 × 10^5 to 1 × 10^7 cells/mL; staining complete within 1–2 minutes of mixing |
| Sensitivity / LOD | Enumeration of dead cells present at ≥1% of total population in a typical counting chamber field |
| Specificity | Specifically stains membrane-damaged dead and dying cells; live cells exclude the dye and appear clear and refractive; does not discriminate apoptotic from necrotic cell death mechanisms |
| Reaction Conditions / Protocol | Mix equal volumes of cell suspension and 0.4% Trypan Blue solution; load 10 μL into hemocytometer chamber; count immediately or within 3 minutes to avoid cytotoxicity artifacts; calculate viability percentage as (live cells / total cells) × 100% |
| Components / Formulation | 0.4% Trypan Blue in 0.81% sodium chloride and 0.06% potassium phosphate dibasic, sterile-filtered; pH 7.2–7.4 |
| Storage Conditions | 15–25°C protected from light; avoid freezing which causes dye precipitation; filter before use if particulate matter is observed; stable at room temperature |
| Shelf Life | 36 months from date of manufacture |
| Package Specifications | 20 mL, 100 mL, 500 mL bottles |
| Product Form | Sterile ready-to-use isotonic solution |
| Quality Control | Each lot verified for osmolarity (280–320 mOsm/kg) and pH (7.2–7.4); sterility tested per USP <71>; endotoxin tested (<0.5 EU/mL); staining efficacy confirmed with HeLa cell reference culture |
| Key Features | Classical dye-exclusion method with decades of published validation; minimum instrumentation required; compatible with manual hemocytometer counting and automated cell counters; negligible cost per test |
For research use only, not for clinical use.
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