| Product Name | 7-AAD Cell Viability Assay Reagent |
| Catalog No. | CATR-HMM-0034 |
| Description | 7-AAD, whose full English name is 7-aminoactinomycin D and Chinese name is 7-aminoactinomycin D, is a fluorescent indicator that can bind to nucleic acids. The DNA complex formed emits maximum fluorescence at 647 nm under 546 nm excitation light, making it commonly used in multicolor fluorescence microscopy or flow cytometry experiments. |
| Application | This product is developed based on the characteristic of the far-red fluorescent probe 7-AAD (7-aminoactinomycin D) specifically staining necrotic cells with lost cell membrane integrity, enabling specific staining of the cell nuclei of necrotic cells to detect cell necrosis. |
| CAS | 7240-37-1 |
| Formula | C62H87N13O16 |
| MW | 1270.45 |
| Spectral Parameters | Ex/Em = 564/647 nm |
| Applicable Instruments | Flow cytometer, fluorescence microscope, or other fluorescence detection equipment |
| Storage | Store at -20°C away from light. |
| Shelf Life | 1 year |
| Notes | If there is bacterial or fungal contamination, it may significantly affect the detection results. It is advisable to perform the assay as soon as possible after staining, as prolonged exposure may lead to an increase in the number of necrotic cells. After fixation, permeabilization, and RNase treatment, 7-AAD can also be used to detect the cell cycle, but propidium iodide (PI) is more commonly used. All fluorescent dyes are subject to photobleaching; please take care to avoid exposure to light to slow down photobleaching. |
For research use only, not for clinical use.
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