| Product Name | EdU (5-Ethynyl-2'-deoxyuridine) |
| Catalog No. | CATR-HMM-0010 |
| Description | EdU, or 5-ethynyl-2’-deoxyuridine (5-Ethynyl-2’ -deoxyuridine), is a thymidine nucleoside analog that can replace thymidine (T) and incorporate into replicating DNA molecules during cell proliferation. By leveraging the specific reaction between EdU and fluorescently labeled dyes, this reagent enables rapid detection of cellular DNA replication activity, thereby providing a fast and accurate assessment of cell proliferation capacity. |
| Application | This product is suitable for live animal injections and for detecting cell proliferation in vitro. |
| CAS | 61135-33-9 |
| Formula | C11H12N2O5 |
| MW | 252.2 |
| Applicable Instruments | Fluorescence microscope, flow cytometer |
| Materials to Bring | 96/24/12/6-well culture plates or culture dishes; 10 mM PBS, pH 7.2–7.6; 4% paraformaldehyde fixative (in PBS); permeabilization reagent (0.5% Triton X-100 in PBS); 2 mg/mL glycine solution (in ddH₂O); 3% BSA in PBS, pH 7.2–7.6; 1% BSA in PBS, pH 7.2–7.6; ddH₂O |
| Storage | The powder should be stored in the dark at -20°C and is valid for one year. When prepared as a solution, it should be stored in the dark at -80°C and is valid for six months. When stored in the dark at -20°C, it is valid for one month. |
| Features | Fast and efficient: Click chemistry reaction, only takes a few minutes to detect cell DNA replication activity and accurately detect cell proliferation capacity; Simple and accurate: No need for DNA denaturation, protease treatment, or overnight incubation of antigens and antibodies, which better protects cell morphology; Wide applicability: Wide range of applications, suitable for cells and various animal live injections; |
| Notes | 1. Before use, centrifuge the product briefly to the bottom of the tube before proceeding with subsequent experiments. 2. This product may cause irreversible damage to the skin. Please take precautions and avoid inhalation risks. |
For research use only, not for clinical use.
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