L-Lactate Dehydrogenase Enzyme for Pyruvate Reduction Assay >=150 U/mg 35 kDa 0.1% BSA Stabilized CAS 9028-36-8

Name: L-Lactate Dehydrogenase Enzyme for Pyruvate Reduction Assay >=150 U/mg 35 kDa 0.1% BSA Stabilized CAS 9028-36-8
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Cat.No: IEC-HMM-0289 Datasheet

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Product Name	L-Lactate Dehydrogenase Enzyme for Pyruvate Reduction Assay >=150 U/mg 35 kDa 0.1% BSA Stabilized CAS 9028-36-8
Catalog No.	IEC-HMM-0289
Description	L-Lactate dehydrogenase from Alta DiagnoTech catalyzes the reversible reduction of pyruvate to L-lactate using NADH. It is widely used in clinical diagnostic kits measuring lactate, pyruvate, and as a coupled indicator enzyme.
Intended Use	Enzyme raw material for lactate/pyruvate assays and coupled diagnostic reagents.
Principle / Technology	Reduces pyruvate to lactate while oxidizing NADH to NAD; the decrease in absorbance at 340 nm correlates with analyte concentration.
Detection Method	NADH absorbance at 340 nm.
Sample Type	Serum, plasma, cerebrospinal fluid.
Performance Range / Specifications	Specific activity >=150 U/mg. Molecular weight 35 kDa. Isoelectric point 4.5.
Components / Formulation	Lactate dehydrogenase lyophilized powder.
Storage Conditions	-20°C.
Shelf Life	Stable for at least 12 months at -20°C.
Package Specifications	KU packaging units.
Product Form	White amorphous powder.
Purity	99%
Activity / Unit Definition	>=150 U/mg
Molecular Weight	35 kDa
Source / Origin	Microbial fermentation.
pH Range / Optimal pH	Optimal pH 6.5-8.0; stable between pH 4.5-10.0 at 25°C for 24 hours.
Shipping Conditions	Cold chain shipment.
Expiration Date / Stability	-20°C stable for one year.
Appearance	White amorphous powder.
CAS Number	9028-36-8
Isoelectric Point	4.5
Inhibitors	Not inhibited by sodium azide.
Optimal Temperature	40-50°C.
Thermal Stability	No loss of activity below 55°C for 15 minutes.
Handling Precaution	Adding 0.1% BSA helps stabilize activity; avoid repeated freeze-thaw.
Michaelis Constant	4.0×10^-4 mol/L (Pyruvate)
Stabilizer	0.1% BSA helps stabilize enzyme activity.

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For research use only, not for clinical use.