| Product Name | Human Platelet Activating Factor (PAF) ELISA Kit |
| Catalog No. | PAK-HMM-0010 |
| Description | Platelet-activating factor, also known as PAF, PAF-acether, or AGEPC (acetylglycerol ether-phosphatidylcholine), is a potent phospholipid activator and mediator of many leukocyte functions, platelet aggregation and degranulation, inflammation, and anaphylaxis. It is also involved in changes in vascular permeability, oxidative bursts, leukocyte chemotaxis, and enhanced arachidonic acid metabolism in phagocytes.PAF is produced by a wide variety of cells, but particularly those involved in host defense, such as platelets, endothelial cells, neutrophils, monocytes, and macrophages.PAF is produced continuously by these cells, but in small amounts, and its production is controlled by the activity of the PAF acetylhydrolase enzyme. Control. Inflammatory cells produce large amounts of PAF in response to specific stimuli. |
| Test Species | Human |
| Synonyms | PAF-Acether; AGEPC; Acetyl-Glyceryl-Ether-Phosphorylcholine |
| Applications | The kit detects human PAF in samples without significant cross-reactivity with its analogs. |
| Sample Type | Serum, plasma and other biological fluids. |
| Detection Methods | Competitive ELISA |
| Sensitivity | 23 pg/mL |
| Detection Range | 46.87-3000 pg/mL |
| Accuracy | The intraplate coefficient of variation is less than 10% and the interplate coefficient of variation is less than 10%. |
| Storage | 2-8°C |
| Shelf Life | 6 months |
| Detection Principle | This kit is a competitive enzyme-linked immunosorbent assay (ELISA). To the microtiter wells pre-coated with human platelet-activating factor (PAF) antigen, the sample, standard, Biotin-labeled antibody, HRP enzyme conjugate are added sequentially, and then the sample is incubated and washed, and then the color is developed with the substrate TMB, which is converted into blue color catalyzed by HRP, and then converted into yellow color under the effect of acid. The shade of color is negatively correlated with human platelet-activating factor (PAF) in the sample. The absorbance (OD value) is measured at 450 nm with an enzyme marker to calculate the sample concentration. |
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