| Product Name | Human Glycoprotein IX (platelet) (GP9) ELISA Kit |
| Catalog No. | PAK-HMM-0015 |
| Description | Glycoprotein IX (platelet) (GP9) is also known as CD42a (cluster of differentiation 42a). It forms a 1-to-1 noncovalent complex with glycoprotein Ib (GP Ib), a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor (known as the glycoprotein Ib-IX-V receptor complex). The main part of the receptor is a heterodimer consisting of 2 polypeptide chains, an alpha chain and a beta chain, which are linked by disulfide bonds. |
| Test Species | Human |
| Synonyms | GP9, CD42a, GPIX, Glycoprotein IX, glycoprotein IX platelet |
| Applications | The kit detects human GP9; CD42α in samples without significant cross-reactivity with its analogs. |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Detection Methods | Sandwich ELISA |
| Sensitivity | 7.4 pg/mL |
| Detection Range | 15.6-1000 pg/mL |
| Accuracy | The intraplate coefficient of variation is less than 10% and the interplate coefficient of variation is less than 10%. |
| Storage | 2-8°C |
| Shelf Life | 6 months |
| Detection Principle | The kit is based on double antibody sandwich enzyme-linked immunosorbent assay (ELISA). To the microtiter wells pre-coated with human platelet glycoprotein IX (GP9; CD42α) capture antibody, the sample, standard, biotin-labeled detection antibody, HRP enzyme conjugate are added sequentially, and then incubated and washed, and then the color is developed with the substrate TMB, which is catalyzed by peroxidase (HRP) to blue, and then converted to the final yellow color under the effect of acid. The shade of color is positively correlated with human platelet glycoprotein IX (GP9; CD42α) in the sample. The absorbance (OD value) is measured at 450 nm with an enzyme marker to calculate the sample concentration. |
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