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| Product Name | Human PDGF-CC ELISA Kit |
| Catalog No. | PAK-HMM-0018 |
| Description | Platelet-derived growth Platelet-derived growth factor CC (PDGF-CC), plays an important role in regulating embryonic development, mesenchymal cell proliferation, migration and survival. |
| Test Species | Human |
| Applications | This kit detects human PDGF-CC in samples without significant cross-reactivity with its analogs. |
| Sample Type | Serum, plasma and cell culture supernatants and other biological fluids. |
| Detection Methods | Sandwich ELISA |
| Sensitivity | 30.2 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Accuracy | The intraplate coefficient of variation is less than 10% and the interplate coefficient of variation is less than 10%. |
| Storage | 2-8°C |
| Shelf Life | 6 months |
| Detection Principle | The kit is based on enzyme-linked immunosorbent assay (ELISA) using double antibody sandwich method. To the microtiter wells pre-coated with human platelet-derived growth factor CC (PDGF-CC) capture antibody, the sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are added sequentially, with warming and washing in between, and the color is developed with the substrate TMB, which is converted to blue color catalyzed by the peroxidase enzyme (HRP), and then converted to the final yellow color under the action of acid. The shade of color is positively correlated with human platelet-derived growth factor CC (PDGF-CC) in the sample. The absorbance (OD value) is measured at 450nm wavelength with an enzyme marker to calculate the concentration of the sample. |
Platelet-derived growth factor CC (PDGF-CC) is a crucial member of the PDGF/VEGF superfamily, playing indispensable roles in multiple biological processes and pathological mechanisms. Unlike other well-characterized PDGF isoforms (such as PDGF-A and PDGF-B), PDGF-CC exhibits unique structural and functional properties that make it a key target for scientific research.
Structural Characteristics: PDGF-CC is initially synthesized and secreted as a latent precursor protein. It contains a distinct N-terminal CUB domain, which must be cleaved by specific proteases (e.g., plasmin or matrix metalloproteinases) to release the active, receptor-binding form. This post-translational activation step is a critical regulatory mechanism that ensures PDGF-CC exerts its biological effects only at the right time and in the appropriate microenvironment. The active form of PDGF-CC consists of two polypeptide chains linked by disulfide bonds, enabling it to bind specifically to its cognate receptors (PDGFR-αα and PDGFR-αβ).
Biological Functions:
Embryonic Development: During embryogenesis, PDGF-CC is essential for the formation and maturation of multiple tissues and organs. It regulates the proliferation, migration, and differentiation of mesenchymal cells, which are the precursors of connective tissues, bones, and blood vessels. For example, PDGF-CC contributes to the development of the central nervous system, cardiovascular system, and skeletal system by guiding the assembly of cell populations and the formation of extracellular matrix.
Tissue Repair and Regeneration: In adult organisms, PDGF-CC is rapidly upregulated at sites of tissue injury (e.g., wound healing, myocardial infarction). It promotes the recruitment of inflammatory cells and fibroblasts to the injured area, stimulates the synthesis of collagen and other extracellular matrix components, and accelerates the formation of new blood vessels (angiogenesis) to support tissue repair and regeneration.
Pathological Roles: Abnormal expression or activation of PDGF-CC is closely associated with various human diseases. In cancer, overexpression of PDGF-CC can drive tumor growth, invasion, and metastasis by promoting the proliferation of cancer-associated fibroblasts, enhancing angiogenesis, and remodeling the tumor microenvironment. It is also involved in the progression of fibrotic diseases (e.g., pulmonary fibrosis, liver fibrosis) by inducing excessive accumulation of extracellular matrix and activation of myofibroblasts. Additionally, PDGF-CC has been implicated in the pathogenesis of neurological disorders, such as spinal cord injury and neurodegenerative diseases, by regulating glial cell activation and neuroinflammation.
Research Significance: Given its diverse biological functions and pathological implications, PDGF-CC has become a focus of scientific research in fields such as cell biology, molecular biology, oncology, immunology, and regenerative medicine. Accurate and sensitive detection of PDGF-CC levels in biological samples (e.g., serum, plasma, cell culture supernatants) is essential for understanding its role in physiological and pathological processes, identifying potential biomarkers for disease diagnosis and prognosis, and evaluating the efficacy of therapeutic strategies targeting PDGF-CC or its signaling pathway. The Human PDGF-CC ELISA Kit provides a reliable tool for researchers to achieve this goal, enabling quantitative analysis of PDGF-CC in various sample types with high specificity and precision.
High Specificity: The kit utilizes a double antibody sandwich ELISA method with highly specific capture and detection antibodies that are designed to recognize human PDGF-CC. It shows no significant cross-reactivity with other PDGF isoforms (e.g., PDGF-A, PDGF-B, PDGF-DD) or related molecules in the VEGF family, ensuring that the detected signal accurately reflects the level of PDGF-CC in the sample.
Wide Detection Range: With a detection range of 62.5 - 4000 pg/mL, the kit can quantify PDGF-CC levels in a broad spectrum of biological samples, from low-concentration samples (e.g., cell culture supernatants from unstimulated cells) to high-concentration samples (e.g., serum from patients with certain diseases). This eliminates the need for excessive sample dilution or concentration, simplifying the experimental workflow and reducing potential errors.
High Sensitivity: The kit has a sensitivity of 30.2 pg/mL, allowing for the detection of low levels of PDGF-CC that may be present in early-stage pathological processes or subtle changes in physiological conditions. This high sensitivity is crucial for researchers studying the role of PDGF-CC in early disease development or in response to subtle experimental manipulations.
Excellent Precision: The intraplate coefficient of variation (CV) is less than 10% and the interplate CV is less than 10%, indicating that the kit produces consistent and reliable results both within the same assay plate and across different assay plates. This high precision ensures that experimental data is reproducible, which is essential for drawing valid scientific conclusions.
Versatile Sample Compatibility: The kit is suitable for detecting human PDGF-CC in a variety of biological samples, including serum, plasma, and cell culture supernatants. It can also be used with other biological fluids (with appropriate validation), providing researchers with flexibility in sample selection and enabling the analysis of PDGF-CC in different physiological and pathological contexts.
Easy-to-Follow Protocol: The kit comes with a detailed and user-friendly protocol that guides researchers through each step of the assay, from sample preparation and reagent preparation to incubation, washing, and detection. The protocol includes clear instructions on incubation times, temperatures, and reagent volumes, making it easy for both experienced and novice researchers to perform the assay successfully.
Stable Storage Conditions: The kit can be stored at 2 - 8°C for a shelf life of 6 months, eliminating the need for expensive ultra-low temperature storage equipment and reducing the risk of reagent degradation during storage and transportation. This stable storage condition also allows for convenient inventory management and ensures that the kit remains functional for an extended period.
Reliable Quantitative Results: Compared to qualitative or semi-quantitative detection methods (e.g., Western blotting), the ELISA kit provides accurate quantitative data on PDGF-CC levels in samples. This allows researchers to precisely measure changes in PDGF-CC expression under different experimental conditions (e.g., treatment with drugs, genetic manipulation) and to compare PDGF-CC levels between different sample groups (e.g., healthy vs. diseased individuals), facilitating the identification of meaningful biological differences.
Time and Labor Efficiency: The kit is designed for high-throughput analysis, with a 48T or 96T format that allows for the simultaneous detection of multiple samples. The assay procedure is streamlined, with minimal hands-on time required (e.g., no need for complex sample separation or gel electrophoresis). This saves researchers valuable time and labor, enabling them to process more samples in a shorter period and to focus on other aspects of their research.
Cost-Effective: The kit offers a cost-effective solution for PDGF-CC detection compared to other advanced detection technologies (e.g., mass spectrometry, immunoassays using labeled antibodies with expensive detectors). It provides high-quality results at a relatively low cost per sample, making it suitable for laboratories with limited research budgets or for large-scale screening experiments that require the analysis of a large number of samples.
Minimal Sample Requirement: The kit requires only a small volume of sample (typically 50 - 100 µL per well) for detection, which is particularly advantageous when working with limited or precious samples (e.g., serum from rare disease patients, cell culture supernatants from primary cells that are difficult to culture in large quantities). This minimizes sample wastage and allows for the reuse of remaining samples for other experiments if needed.
Validated Performance: The kit has undergone rigorous performance validation, including tests for specificity, sensitivity, precision, accuracy, and linearity. The validation data ensures that the kit meets high-quality standards and can produce reliable results in different laboratory settings. Researchers can have confidence in the data generated using this kit, reducing the risk of experimental errors and the need for repeated experiments.
Support for Multiple Research Applications: The kit is suitable for a wide range of research applications, including basic research on PDGF-CC biology, preclinical studies evaluating the efficacy of PDGF-CC-targeted therapeutics, and clinical research investigating the potential of PDGF-CC as a disease biomarker. Its versatility makes it a valuable tool for researchers in various fields of life sciences.
For research use only, not for clinical use.
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