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| Product Name | Hot-Start Super Specific Taq DNA Polymerase (5 U/μL, for Genotyping) |
| Catalog No. | IVDRM-0265 |
| Description | This hot-start Taq DNA polymerase is blocked with a proprietary antibody that suppresses the 5'→3' polymerase activity during reaction setup and ramp-up, preventing non-specific amplification. The blocking antibody is fully inactivated by a 30-second heating step at the pre-denaturation temperature, releasing full DNA polymerase activity. The enzyme delivers high amplification efficiency, specificity, and sensitivity, and is well suited for Amplification Refractory Mutation System (ARMS) PCR and other high-specificity PCR applications. |
| Intended Use | Designed for SNP genotyping, ARMS-PCR, and other high-specificity PCR amplification in molecular diagnostic assay development. |
| Principle / Technology | Antibody-mediated hot-start technology: a specific antibody blocks the 5'→3' polymerase activity of Taq DNA polymerase at low temperatures. A 30-second incubation at the pre-denaturation temperature irreversibly inactivates the antibody and restores full polymerase activity. |
| Detection Method | PCR / ARMS-PCR (endpoint or real-time amplification-based detection) |
| Sample Type | Purified DNA templates, including mixed samples containing low proportions of mutant allele against a wild-type background |
| Performance Range / Specifications | Strong specificity for SNP genotyping In an ARMS-PCR premix, reliably detects 0.05% mutant template while showing no amplification of 100% wild-type template |
| Sensitivity / LOD | Detects mutant template present at 0.05% against a wild-type background in ARMS-PCR |
| Specificity | High; no amplification observed with 100% wild-type template in ARMS-PCR genotyping assays |
| Reaction Conditions / Protocol | Hot-start activation: heat at the pre-denaturation temperature for 30 sec to fully inactivate the blocking antibody and release polymerase activity; then proceed with standard PCR cycling. |
| Components / Formulation | Antibody-blocked hot-start Taq DNA polymerase, 5 U/μL |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 1 year when stored at -25°C to -15°C |
| Package Specifications | 250 U 500 U 1 KU 10 KU 25 KU 100 KU |
| Product Form | Liquid |
| Concentration | 5 U/μL |
| Application Notes / Precautions | Ensure a pre-denaturation step of at least 30 sec for complete hot-start activation. |
For research use only, not for clinical use.
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