Hot-Start J-Taq DNA Polymerase (5 U/μL, for Multiplex PCR)

Name: Hot-Start J-Taq DNA Polymerase (5 U/μL, for Multiplex PCR)
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Cat.No: IVDRM-0240 Datasheet

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Product Name	Hot-Start J-Taq DNA Polymerase (5 U/μL, for Multiplex PCR)
Catalog No.	IVDRM-0240
Description	This product is an antibody-blocked, thermostable mutant Taq DNA polymerase. Its polymerase activity remains inhibited at 60 °C and is released only after heating at 95 °C, thereby preventing non-specific amplification and primer-dimer formation during sample preparation and reaction ramp-up. Like wild-type Taq DNA polymerase, J-Taq possesses 5'->3' polymerase activity and 5'->3' exonuclease activity, but lacks 3'->5' exonuclease (proofreading) activity.
Intended Use	Raw material enzyme for high-sensitivity PCR and multiplex PCR assay development in molecular diagnostics.
Principle / Technology	Antibody-based hot-start technology: a blocking antibody suppresses polymerase activity at ambient temperature and up to 60 °C; activity is released by 95 °C heating during initial denaturation.
Detection Method	PCR / real-time PCR
Performance Range / Specifications	High specificity: antibody blocking suppresses polymerase activity at room temperature, improving reaction specificity. No nuclease residue: free of exonuclease, endonuclease, and RNase contamination. Suitable for high-sensitivity PCR and multiplex PCR experiments.
Specificity	Antibody blocking suppresses polymerase activity at ambient temperature, effectively preventing non-specific amplification and primer-dimers during reaction setup and ramp-up.
Reaction Conditions / Protocol	Hot-start activation: polymerase activity remains inhibited at 60 °C and is released after heating at 95 °C (initial denaturation step).
Components / Formulation	Antibody-blocked mutant Taq DNA polymerase supplied at 5 U/uL.
Storage Conditions	Store at -25 to -15 °C.
Shelf Life	2 years
Package Specifications	250 U; 500 U; 1000 U; 10000 U; 25000 U
Product Form	Liquid enzyme solution, 5 U/uL
Quality Control	Tested free of exonuclease, endonuclease, and RNase residue.
Purity	No detectable exonuclease, endonuclease, or RNase residue.
Concentration	5 U/uL
Source / Origin	Recombinant thermostable mutant Taq DNA polymerase expressed in E. coli
Expiration Date / Stability	Stable for 2 years at -25 to -15 °C.
Compatibility	Suitable for high-sensitivity PCR and multiplex PCR applications.
Application Notes / Precautions	Possesses 5'->3' polymerase and 5'->3' exonuclease activities; lacks 3'->5' exonuclease (proofreading) activity, as with wild-type Taq DNA polymerase.
Heat Inactivation	Not applicable (thermostable enzyme); the hot-start blocking antibody is inactivated by heating at 95 °C to release polymerase activity.

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For research use only, not for clinical use.