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| Product Name | Hot-Start J-Taq DNA Polymerase (40 U/μL, Glycerol-Free) |
| Catalog No. | IVDRM-0267 |
| Description | This hot-start DNA polymerase is supplied as a high-concentration, glycerol-free formulation. It is blocked with a proprietary antibody and delivers good amplification sensitivity and specificity. A 30-second heating step at the pre-denaturation temperature fully inactivates the blocking antibody and releases DNA polymerase activity. The hot-start mechanism effectively suppresses amplification caused by non-specific primer annealing. The glycerol-free, high-concentration format is designed for the preparation of lyophilized PCR systems, reducing cost and operational complexity. |
| Intended Use | For the development and manufacture of lyophilized (freeze-dried) PCR/qPCR reagents, including in-situ lyophilization and lyophilized microbead formats; also suitable for conventional liquid PCR systems. |
| Principle / Technology | Antibody-mediated hot-start technology: the blocking antibody suppresses Taq DNA polymerase activity at low temperatures and is fully inactivated by a 30-second incubation at the pre-denaturation temperature, releasing polymerase activity. |
| Detection Method | PCR / real-time PCR (qPCR) |
| Sample Type | Purified DNA templates |
| Performance Range / Specifications | Suitable for the preparation of lyophilized systems, reducing cost and operational complexity Supports customized lyophilization formulations Reference lyophilization process available to support freeze-drying workflow development Post-lyophilization performance is consistent with the liquid reagent, with full retention of enzyme activity Stable at room temperature after lyophilization, enabling ambient shipping and storage |
| Sensitivity / LOD | Good amplification sensitivity and specificity |
| Specificity | Antibody hot-start blocking effectively suppresses amplification caused by non-specific primer annealing |
| Reaction Conditions / Protocol | Hot-start activation: heat at the pre-denaturation temperature for 30 sec to fully inactivate the blocking antibody; then proceed with standard PCR cycling. |
| Components / Formulation | Antibody-blocked hot-start J-Taq DNA polymerase, 40 U/μL, glycerol-free |
| Storage Conditions | Store at 2-8°C. |
| Shelf Life | 2 years when stored at 2-8°C |
| Package Specifications | 2 KU 20 KU 80 KU 400 KU |
| Product Form | Liquid, glycerol-free, high-concentration formulation; suitable for lyophilization (in-situ lyophilization and lyophilized microbeads) |
| Concentration | 40 U/μL |
| Shipping Conditions | After lyophilization, the formulated reagent can be shipped and stored at room temperature |
| Application Notes / Precautions | Glycerol-free formulation intended for lyophilization workflows; reference lyophilization protocols and customized formulation support are available. |
| Batch-to-Batch Consistency | Performance after lyophilization is consistent with the liquid reagent, with full retention of enzyme activity |
For research use only, not for clinical use.
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