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| Product Name | Hot-Start High-Tolerant Taq DNA Polymerase, Ultra-Clean (20 U/μL) |
| Catalog No. | IVDRM-0268 |
| Description | This hot-start DNA polymerase is dual-blocked with two proprietary antibodies that suppress both the 5'→3' polymerase activity and the 5'→3' exonuclease activity of Taq DNA polymerase. It is an optimal DNA polymerase for PCR amplification of uracil-containing template DNA after bisulfite conversion. A 30-second heating step at the pre-denaturation temperature fully inactivates the blocking antibodies, releasing both polymerase and exonuclease activities. The dual-blocking design prevents non-specific amplification caused by mispriming or primer dimers and suppresses fluorescence signal decay caused by probe degradation, improving the stability of in vitro diagnostic reagents during shipping and room-temperature handling. Manufactured to an ultra-clean, low nucleic acid background standard, with residual E. coli genomic DNA controlled to ≤1 copy/μL. |
| Intended Use | For methylation detection assays and melt curve analysis; optimal for PCR amplification of bisulfite-treated, uracil-containing DNA templates in epigenetic diagnostic applications. |
| Principle / Technology | Dual-antibody hot-start technology: two blocking antibodies suppress the 5'→3' polymerase and 5'→3' exonuclease activities at low temperatures; both activities are restored after a 30-second incubation at the pre-denaturation temperature. |
| Detection Method | PCR / real-time PCR (qPCR), including methylation-specific PCR and melt curve analysis |
| Sample Type | Bisulfite-converted DNA templates containing uracil; low-methylation-level samples |
| Performance Range / Specifications | Suitable for methylation detection projects; tolerates bisulfite-treated templates Ultra-clean, low nucleic acid background: residual E. coli genomic DNA ≤1 copy/μL In PCR premix testing of a commercial template with 0.5% methylation rate, showed better amplification than comparable products, with improved curve peak shape and detection advantage at low template concentrations |
| Sensitivity / LOD | Detects templates with 0.5% methylation rate; advantageous amplification of low-concentration templates with better peak shape |
| Specificity | Dual antibody blocking prevents non-specific amplification from mispriming and primer dimers and suppresses probe degradation-related fluorescence signal loss |
| Reaction Conditions / Protocol | Hot-start activation: heat at the pre-denaturation temperature for 30 sec to fully inactivate the blocking antibodies; then proceed with standard PCR cycling. |
| Components / Formulation | Dual-antibody-blocked hot-start Taq DNA polymerase, 20 U/μL; ultra-clean, low nucleic acid background (residual E. coli gDNA ≤1 copy/μL) |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 1 year when stored at -25°C to -15°C |
| Package Specifications | 1,000 U 10,000 U 20,000 U 100,000 U |
| Product Form | Liquid |
| Quality Control | Residual E. coli genomic DNA ≤1 copy/μL (ultra-clean, low nucleic acid background) |
| Purity | Residual host (E. coli) genomic DNA ≤1 copy/μL |
| Concentration | 20 U/μL |
| Methylation Sensitivity | Suitable for methylation detection; amplifies bisulfite-treated, uracil-containing templates; detects 0.5% methylation rate templates |
For research use only, not for clinical use.
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