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| Product Name | Hot-Start E-Taq DNA Polymerase (5 U/μL, Glycerol-Free) |
| Catalog No. | IVDRM-0264 |
| Description | This hot-start DNA polymerase employs a proprietary dual-antibody double-blocking mechanism. Both the 5'→3' polymerase activity and the 5'→3' exonuclease activity of Taq DNA polymerase are blocked at low temperatures; upon heating at the pre-denaturation temperature for 30 sec, the blocking antibodies are completely inactivated, releasing both polymerase and exonuclease activities. The double-blocking feature not only effectively prevents non-specific amplification caused by mis-priming or primer dimers, but also suppresses fluorescence signal decay caused by probe degradation, providing dual protection that keeps in vitro diagnostic reagents more stable during transport and room-temperature handling. The product contains no glycerol and is specifically designed for the preparation of lyophilized reagents. |
| Intended Use | Preparation of lyophilized PCR/qPCR reagents; high-sensitivity detection of low-copy-number targets; hot-start PCR and probe-based qPCR assay development. |
| Principle / Technology | Dual-antibody hot start. Two proprietary monoclonal antibodies simultaneously block the 5'→3' polymerase and 5'→3' exonuclease activities of Taq DNA polymerase at low temperatures, preventing mis-priming, primer-dimer formation and probe degradation before the reaction starts. Heating at the pre-denaturation temperature for 30 sec fully inactivates both antibodies, releasing polymerase and exonuclease activities for amplification. |
| Sample Type | DNA/cDNA templates, including low-copy-number targets |
| Performance Range / Specifications | Suitable for lyophilized system formulation, reducing cost and operational complexity Customized lyophilization formulations supported Reference lyophilization process available to facilitate lyophilization workflows Performance after lyophilization is identical to the liquid reagent, with enzyme activity fully retained Stable at room temperature after lyophilization, allowing ambient shipping and storage |
| Sensitivity / LOD | High sensitivity; equivalent amplification performance demonstrated down to 10^3 copies/mL templates in qPCR |
| Specificity | Dual blocking of polymerase and exonuclease activities effectively prevents non-specific amplification from mis-priming or primer dimers and suppresses fluorescence signal decay from probe degradation |
| Storage Conditions | Store at 2-8 °C |
| Shelf Life | 1 year at 2-8 °C |
| Package Specifications | 500 U; 1000 U; 10 KU; 50 KU; 100 KU |
| Product Form | Liquid, glycerol-free (lyophilization-ready) |
| Concentration | 5 U/uL |
| Expiration Date / Stability | Stable performance after accelerated aging at 37 °C for 54 days; amplification performance identical to non-accelerated control |
| Compatibility | Compatible with PCR and probe-based qPCR systems; designed for lyophilized reagent preparation |
| Application Notes / Precautions | Pre-denaturation heating for 30 sec fully inactivates the blocking antibodies and activates the enzyme Amplification performance of the glycerol-free enzyme is equivalent to the corresponding glycerol-containing enzyme Lyophilized product remains stable during room-temperature transport and storage |
For research use only, not for clinical use.
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