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| Product Name | Homing Endonuclease I-CeuI (Recognition Site: 5'-TAACTATAACGGTCCTAA↓GGTAGCGAA-3') |
| Catalog No. | IVDRM-0227 |
| Description | I-CeuI is a homing endonuclease originally derived from Chlamydomonas eugametos and obtained by recombinant expression and purification from E. coli. It specifically recognizes and cleaves a 27-bp non-palindromic sequence (5'-TAACTATAACGGTCCTAAGGTAGCGAA-3'), generating cohesive ends with a 4-bp 3' overhang. Owing to its exceptionally long recognition site, which occurs only once in approximately 5.6 x 10^17 random bases, I-CeuI offers even higher specificity than I-SceI in gene editing applications. |
| Intended Use | For gene editing, genome engineering, and molecular biology research applications. For research use only. |
| Principle / Technology | Homing endonuclease (meganuclease); sequence-specific cleavage of an ultra-long 27-bp non-palindromic target, generating a 4-bp 3' protruding cohesive end. |
| Detection Method | Cleavage products are typically analyzed by agarose gel electrophoresis. |
| Sample Type | Double-stranded DNA containing the I-CeuI recognition site (e.g., plasmid or genomic DNA substrates). |
| Specificity | No isoschizomers. Recognizes a 27-bp non-palindromic sequence with an estimated natural occurrence of once per ~5.6 x 10^17 random bases, providing extremely high specificity for gene editing. |
| Reaction Conditions / Protocol | 1x universal fast-digestion buffer; incubate at 37°C. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years when stored as recommended. |
| Package Specifications | 250 U |
| Concentration | 5 U/uL |
| Activity / Unit Definition | One unit (U) is defined as the amount of enzyme required to digest 1 ug of pUC-HE DNA in 1 hour at 37°C. |
| Source / Origin | Derived from Chlamydomonas eugametos; recombinantly expressed and purified from E. coli. |
| Expiration Date / Stability | Stable for 2 years at -25°C to -15°C. |
| Recommended Buffer System | 1x universal fast-digestion buffer |
| Recognition Site | 5'-TAACTATAACGGTCCTAA↓GGTAGCGAA-3' 3'-ATTGATATTGCCAG↑GATTCCATCGCTT-5' |
| Heat Inactivation | Incubate at 80°C for 20 min. |
| Methylation Sensitivity | No known effect of methylation. |
For research use only, not for clinical use.
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