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| Product Name | High-Specificity Multiplex Hot-Start Taq DNA Polymerase |
| Catalog No. | IEC-HMM-0097 |
| Description | An antibody-modified hot-start Taq DNA polymerase system optimized for highly specific, uniform and high-yield multiplex PCR. |
| Intended Use | Multiplex PCR; high-specificity multi-target amplification; direct amplification from compatible blood and blood-card samples. |
| Principle / Technology | Antibody-mediated hot-start control suppresses low-temperature polymerase activity, while an optimized multiplex buffer supports efficient extension of multiple primer pairs in one reaction. |
| Detection Method | Endpoint multiplex PCR with downstream agarose-gel or assay-specific detection. |
| Sample Type | Purified DNA, human genomic DNA and compatible direct-amplification samples such as whole blood or blood cards. |
| Performance Range / Specifications | 200 or 1,000 reactions at 25 µL per reaction; tested with up to 25 targets ranging from 70 to 2,790 bp; compatible with approximately 25%–75% target GC content and diverse primer melting temperatures. |
| Specificity | Designed to reduce nonspecific amplification caused by primer mismatch and to improve target-band uniformity. |
| Reaction Conditions / Protocol | Prepare the multiplex reaction with the supplied 2× buffer and polymerase. A 60°C universal annealing condition is suitable for many assays, while direct-amplification samples may require an extended initial denaturation step. |
| Components / Formulation | 2× high-specificity multiplex PCR buffer and high-specificity multiplex DNA polymerase at 10 U/µL. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions; 1,000 reactions. |
| Product Form | Two-component multiplex hot-start DNA polymerase system. |
| Quality Control | Multiplex yield, target uniformity, GC compatibility, specificity and inhibitor tolerance are evaluated. |
| Key Features | High target specificity; uniform multi-target amplification; high product yield; broad GC and primer-Tm compatibility; direct-amplification compatibility. |
| Concentration | DNA polymerase: 10 U/µL. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for multi-target PCR assays using diverse primer sets and compatible purified or direct-amplification DNA samples. |
| Application Notes / Precautions | Primer quality and concentration strongly influence multiplex balance. Optimize individual primer amounts if selected amplicons are underrepresented. |
For research use only, not for clinical use.
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