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| Product Name | High-Sensitivity Multiplex Hot-Start Taq DNA Polymerase |
| Catalog No. | IEC-HMM-0098 |
| Description | A high-sensitivity antibody-modified hot-start Taq DNA polymerase system developed for robust high-plex PCR and low-template detection. |
| Intended Use | High-plex PCR enrichment; multiplex target amplification; direct amplification from compatible whole-blood and blood-card samples. |
| Principle / Technology | Antibody-mediated hot-start regulation and a multiplex-optimized buffer improve low-template amplification, target balance and resistance to common sample inhibitors. |
| Detection Method | Endpoint multiplex PCR with downstream electrophoretic or assay-specific detection. |
| Sample Type | Purified DNA, genomic DNA, lambda DNA and compatible direct-amplification samples including whole blood and blood cards. |
| Performance Range / Specifications | 200 or 1,000 reactions at 25 µL per reaction; supports target sizes of approximately 50–3,500 bp, broad target GC content of about 25%–75% and high-plex enrichment involving hundreds of amplicons. |
| Sensitivity / LOD | Reported detection reached 0.1 ng in a human genomic system and 0.1 pg in a lambda-DNA system. |
| Specificity | Hot-start control and optimized reaction chemistry reduce nonspecific amplification in complex multiplex assays. |
| Reaction Conditions / Protocol | Combine the supplied 2× multiplex buffer with polymerase, primers and template. A 60°C universal annealing condition is suitable for many assays; extend initial denaturation for direct blood or blood-card amplification when required. |
| Components / Formulation | 2× high-sensitivity multiplex PCR buffer and high-sensitivity multiplex DNA polymerase at 10 U/µL. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions; 1,000 reactions. |
| Product Form | Two-component high-sensitivity multiplex hot-start polymerase system. |
| Quality Control | Low-template sensitivity, high-plex enrichment, inhibitor tolerance, target-size compatibility and accelerated stability are evaluated. |
| Key Features | High sensitivity; high-plex capability; strong inhibitor tolerance; broad amplicon-size and GC compatibility; stable performance under transport and freeze–thaw stress. |
| Concentration | DNA polymerase: 10 U/µL. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Performance remained stable in reported accelerated storage, repeated freeze–thaw and transport evaluations. |
| Compatibility | Compatible with purified DNA and selected direct-amplification samples such as whole blood and blood cards. |
| Application Notes / Precautions | Use high-quality primers and templates. For poorly represented targets, optimize primer concentration, annealing temperature, cycle number or extension time. |
For research use only, not for clinical use.
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