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| Product Name | High-Sensitivity Hot-Start Taq DNA Polymerase |
| Catalog No. | IEC-HMM-0091 |
| Description | A high-sensitivity hot-start Taq DNA polymerase system designed for strong amplification linearity, low-copy detection and stable primer/probe premixing. |
| Intended Use | Hot-start PCR and qPCR; low-copy gene amplification; direct blood SNP genotyping; multiplex pathogen detection. |
| Principle / Technology | Antibody-mediated hot-start control suppresses nonspecific activity during reaction setup and temperature ramping, while the optimized buffer supports sensitive amplification. |
| Detection Method | PCR or probe/dye-based qPCR, depending on assay design. |
| Sample Type | Purified DNA, genomic DNA, cDNA and compatible blood-derived templates. |
| Performance Range / Specifications | 500, 1,000 or 5,000 U; enzyme concentration 5 U/µL; optimized for strong amplification curves and improved low-template detection. |
| Sensitivity / LOD | Enhanced low-copy target detection compared with conventional hot-start amplification systems in the reported evaluation. |
| Specificity | Hot-start control and optimized buffer reduce nonspecific amplification. |
| Reaction Conditions / Protocol | Use the supplied enzyme, Mg²⁺-containing 10× buffer and dNTP mix in a PCR or qPCR formulation appropriate for the target assay. |
| Components / Formulation | Hot-start Taq DNA polymerase, 10× Mg²⁺-containing reaction buffer and dNTP mix. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 500 U; 1,000 U; 5,000 U. |
| Product Form | Hot-start Taq DNA polymerase system. |
| Quality Control | Amplification linearity, sensitivity, detection rate, multiplex performance and accelerated stability are evaluated. |
| Key Features | High sensitivity; strong amplification linearity; stable primer/probe premixing; improved detection rate; multiplex compatibility. |
| Concentration | 5 U/µL. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for PCR and qPCR systems, including low-copy, blood-derived and multiplex assays. |
For research use only, not for clinical use.
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