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| Product Name | High-Purity Deoxyribonuclease I for DNA Degradation, >=95% by SDS-PAGE |
| Catalog No. | IEC-HMM-0179 |
| Description | Alta DiagnoTech deoxyribonuclease I is a high-purity 35 kDa glycoprotein nuclease supplied as white amorphous powder. |
| Intended Use | Enzymatic degradation of DNA and DNase activity measurement using a calf-thymus DNA substrate. |
| Principle / Technology | DNase I converts DNA into oligonucleotides, producing an activity-proportional increase in absorbance at 260 nm. |
| Detection Method | Kunitz absorbance assay at 260 nm |
| Performance Range / Specifications | Specific activity is at least 20,000 Kunitz units/mg protein; assay linear range is 400-500 U/mL. |
| Reaction Conditions / Protocol | A 3.00 mL assay contains 83 mM sodium acetate at pH 5.0, 4.2 mM MgSO4, 25 mM NaCl, 33.33 ug/mL calf-thymus DNA and 200-250 Kunitz units of DNase I. Preincubate 2.5 mL reagent for 1.5 minutes at 25 degrees C, add 0.5 mL sample and record the maximum absorbance-change rate within 2 minutes. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 2 years at -20 degrees C |
| Product Form | White amorphous powder |
| Purity | >=95% by SDS-PAGE; free of RNase, protease and other DNA endonuclease or exonuclease activity |
| Activity / Unit Definition | One Kunitz unit increases A260 by 0.001 per minute at pH 5.0 and 25 degrees C using calf-thymus DNA. |
| Molecular Weight | Approximately 35 kDa glycoprotein |
| pH Range / Optimal pH | Stable at pH 4.0-9.0 for 24 hours at 25 degrees C; optimum pH 6.0 |
| Appearance | White amorphous powder |
| Alias | Deoxyribonuclease I |
| Product Type | Deoxyribonuclease I enzyme raw material |
| Function / Efficacy | Hydrolyze DNA into oligonucleotides. |
| Abbreviation | DNase I |
| Customization | Specifications can be customized |
| Specific Activity | >=20,000 Kunitz units/mg protein |
| Isoelectric Point | Approximately 6.0 |
| Inhibitors | EDTA, EGTA, SDS, DTT, beta-mercaptoethanol and other reducing agents |
| Optimal Temperature | Activity increases from 20 to 60 degrees C; the value at 37 degrees C is 3.3 times the value at 25 degrees C |
| Thermal Stability | Stable below 55 degrees C; retains more than 50% activity after 30 minutes at 60 degrees C; inactive above 70 degrees C |
| Working Solution | Dilute the reconstituted enzyme to 200-500 Kunitz units/mL in 0.85% NaCl before activity measurement. |
| Activator | Mg2+, Mn2+, Ca2+, Co2+ and Zn2+ |
| Detection Wavelength | 260 nm |
| Linear Range | 400-500 U/mL |
For research use only, not for clinical use.
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