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| Product Name | High-Affinity RNase Inhibitor, Porcine (40 U/μL) |
| Catalog No. | IVDRM-0279 |
| Description | High-Affinity RNase Inhibitor is a recombinant porcine RNase inhibitor expressed and purified from E. coli. It forms a non-covalent complex with RNases to inactivate them and broadly inhibits various RNases, including RNase A, RNase B and RNase C. Verified by RT-PCR and RT-qPCR, it is compatible with high-performance reverse transcriptases and various DNA polymerases. Manufactured with an ultra-clean, low nucleic acid background process, it features lower host residuals and is well suited for applications with stricter background requirements, such as pathogen detection. The NP-40-free storage buffer with very low DTT makes it especially suitable for single-cell and low-input RNA library preparation. |
| Intended Use | Protection of RNA from RNase degradation in RT-qPCR, NGS RNA library preparation, single-cell and low-input RNA sequencing workflows, and molecular diagnostic assays such as pathogen detection. |
| Principle / Technology | The porcine inhibitor binds RNases with high affinity through non-covalent interactions, forming an inactive complex that blocks ribonuclease activity and preserves RNA integrity during reverse transcription, amplification and library construction. |
| Detection Method | RT-PCR, RT-qPCR, NGS RNA library preparation |
| Performance Range / Specifications | Storage buffer free of NP-40 with very low DTT concentration, especially suitable for single-cell and low-input RNA library preparation Ultra-clean, low nucleic acid background: E. coli DNA residual < 0.1 copies per 100 U; free of detectable exonuclease, nicking enzyme and RNase residuals Compatible with RT-qPCR systems: no impact on the detection system at an input of 20 U per test Compatible with NGS systems: supports high library yields with low host DNA residuals |
| Sensitivity / LOD | E. coli genomic DNA residual < 0.1 copies/100 U |
| Specificity | Broadly inhibits RNase A, RNase B and RNase C with high affinity. Of porcine (swine) origin, distinguishing it from the conventional murine RNase inhibitor; host genomic DNA residuals are significantly lower than those of the conventional murine version, and 8 U per test effectively inhibits 50 ng of RNase A. |
| Reaction Conditions / Protocol | Compatible with RT-qPCR at an input of up to 20 U per test without affecting the detection system; suitable for NGS RNA library preparation. |
| Components / Formulation | Enzyme storage buffer: 20 mM HEPES-KOH, 150 mM KCl, 8 mM DTT, 0.01% Triton X-100, 50% glycerol, pH 7.5 @ 25 °C; NP-40-free. |
| Storage Conditions | Store at -25 °C to -15 °C. |
| Shelf Life | 2 years |
| Package Specifications | 2 KU; 20 KU; 100 KU; 5.5 KU |
| Product Form | Liquid |
| Quality Control | E. coli genomic DNA residual < 0.1 copies/100 U Free of detectable exonuclease, nicking enzyme and RNase residuals RNase A inhibition and RT-qPCR compatibility functionally verified |
| Concentration | 40 U/μL |
| Activity / Unit Definition | 8 U per test effectively inhibits 50 ng of RNase A. |
| Source / Origin | Recombinant porcine (swine) RNase inhibitor expressed and purified from E. coli. |
| pH Range / Optimal pH | pH 7.5 @ 25 °C (storage buffer) |
| Expiration Date / Stability | Stable for 2 years when stored at -25 °C to -15 °C. |
| Regulatory / Compliance | IVD raw material for molecular diagnostic reagent manufacturing; for research and manufacturing use only. |
| Compatibility | Compatible with reverse transcriptases and various DNA polymerases in RT-PCR/RT-qPCR; compatible with NGS RNA library preparation workflows. |
| Recommended Buffer System | 20 mM HEPES-KOH, 150 mM KCl, 8 mM DTT, 0.01% Triton X-100, 50% glycerol, pH 7.5 @ 25 °C. |
| Application Notes / Precautions | NP-40-free formulation with very low DTT; particularly recommended for single-cell and low-input RNA library preparation. |
For research use only, not for clinical use.
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