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| Product Name | Heat-Labile Uracil-DNA Glycosylase |
| Catalog No. | IEC-HMM-0082 |
| Description | A heat-sensitive uracil-DNA glycosylase for preventing carryover contamination in amplification workflows that use dUTP-containing products. |
| Intended Use | Removal of dU-containing PCR aerosol contamination and uracil bases from single- or double-stranded DNA. |
| Principle / Technology | The enzyme hydrolyzes the N-glycosidic bond of uracil in DNA, generating abasic sites that are readily cleaved during heating. |
| Detection Method | Used as a pre-PCR contamination-control enzyme in PCR, qPCR, RT-PCR and RT-qPCR workflows. |
| Sample Type | Single- and double-stranded DNA containing uracil. |
| Performance Range / Specifications | Irreversibly inactivated at 55°C for 10 minutes. |
| Specificity | Selective for uracil-containing DNA. |
| Reaction Conditions / Protocol | Treat the reaction with the enzyme before amplification and use a 55°C, 10-minute step for irreversible inactivation. |
| Components / Formulation | Heat-labile uracil-DNA glycosylase at 1 U/μL. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 100 U (100 μL); 500 U (500 μL). |
| Product Form | Heat-labile enzyme solution. |
| Key Features | Rapid heat inactivation; high activity in common PCR buffers; supports PCR, qPCR, RT-PCR and RT-qPCR carryover-control workflows. |
| Concentration | 1 U/μL. |
| Activity / Unit Definition | Supplied as 100 U or 500 U total enzyme activity. |
| Source / Origin | Derived from a psychrophilic marine bacterium. |
| Compatibility | Active in a wide range of common PCR buffer systems. |
For research use only, not for clinical use.
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