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| Product Name | Heat-Labile Uracil-DNA Glycosylase, Glycerol-Free |
| Catalog No. | IEC-HMM-0081 |
| Description | A glycerol-free, heat-labile uracil-DNA glycosylase designed for dU-containing DNA carryover control and lyophilized reagent development. |
| Intended Use | Removal of dU-containing PCR aerosol contamination and uracil bases from single- or double-stranded DNA. |
| Principle / Technology | Uracil-DNA glycosylase excises uracil from DNA, creating abasic sites that are subsequently cleaved during heating. |
| Detection Method | Used as a pre-PCR contamination-control enzyme in PCR, qPCR, RT-PCR and RT-qPCR workflows. |
| Sample Type | Single- and double-stranded DNA containing uracil. |
| Performance Range / Specifications | Active at room temperature; most activity is irreversibly inactivated at 50°C for 2 minutes and complete inactivation is achieved at 50°C for 5 minutes. |
| Specificity | Targets uracil-containing DNA while supporting common amplification workflows. |
| Reaction Conditions / Protocol | Add before amplification for dU-containing carryover control, then heat at 50°C for 2–5 minutes to inactivate the enzyme before PCR cycling. |
| Components / Formulation | Glycerol-free heat-labile uracil-DNA glycosylase without additional lyophilization-interfering components. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 1,000 U; 4,000 U. |
| Product Form | Glycerol-free heat-labile enzyme. |
| Key Features | Glycerol-free formulation; suitable for lyophilization; rapid heat inactivation; compatible with PCR, qPCR, RT-PCR and RT-qPCR; strong dU-DNA removal. |
| Shipping Conditions | Ship at 0°C or below. |
| Expiration Date / Stability | Reported to maintain dU-template degradation performance before and after lyophilization. |
| Compatibility | Compatible with common PCR, qPCR, RT-PCR and RT-qPCR reaction systems. |
For research use only, not for clinical use.
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