Glycerol Kinase Enzyme for Triglyceride Glycerol Assay >=20 U/mg 50 kDa Isoelectric Point 4.3 CAS 9030-66-4

Name: Glycerol Kinase Enzyme for Triglyceride Glycerol Assay >=20 U/mg 50 kDa Isoelectric Point 4.3 CAS 9030-66-4
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Cat.No: IEC-HMM-0286 Datasheet

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Product Name	Glycerol Kinase Enzyme for Triglyceride Glycerol Assay >=20 U/mg 50 kDa Isoelectric Point 4.3 CAS 9030-66-4
Catalog No.	IEC-HMM-0286
Description	Glycerol kinase from Alta DiagnoTech phosphorylates glycerol to glycerol-3-phosphate using ATP. It is an essential enzyme in triglyceride and free glycerol determination reagents.
Intended Use	Raw enzyme for triglyceride assay kits, free glycerol tests, and lipid metabolism research.
Principle / Technology	Catalyzes ATP-dependent phosphorylation of glycerol; the product is coupled to NADH-producing or peroxide-producing reactions for quantification.
Detection Method	Spectrophotometric coupled assay.
Sample Type	Serum, plasma.
Performance Range / Specifications	Specific activity >=20 U/mg. Molecular weight 50 kDa. Isoelectric point 4.3.
Components / Formulation	Glycerol kinase lyophilized powder.
Storage Conditions	-20°C.
Shelf Life	Stable for at least 12 months at -20°C.
Package Specifications	KU packaging units.
Product Form	Pale yellow amorphous powder.
Purity	99%
Activity / Unit Definition	>=20 U/mg
Molecular Weight	50 kDa
Source / Origin	Microbial fermentation.
pH Range / Optimal pH	Optimal pH 8.0; stable between pH 6.0-10.0.
Shipping Conditions	Cold chain shipment.
Expiration Date / Stability	-20°C stable for one year.
Appearance	Pale yellow amorphous powder.
CAS Number	9030-66-4
Isoelectric Point	4.3
Inhibitors	Not inhibited by sodium azide.
Optimal Temperature	50°C.
Thermal Stability	Stable below 60°C.
Handling Precaution	Reconstitute in buffer containing Mg2+ or other suitable activators before use.
Michaelis Constant	ATP 1.3×10^-5 M; Glycerol 5.2×10^-5 M

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For research use only, not for clinical use.