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| Product Name | Glycerol-Free Warm-Start RT-LAMP Reverse Transcriptase |
| Catalog No. | IEC-HMM-0107 |
| Description | A thermostable glycerol-free reverse transcriptase engineered for warm-start RT-LAMP and lyophilized isothermal amplification reagents. |
| Intended Use | Reverse transcription loop-mediated isothermal amplification and development of freeze-dried RT-LAMP reagents. |
| Principle / Technology | The enzyme is blocked below approximately 45°C and rapidly activates above 45°C, enabling room-temperature reaction setup and efficient reverse transcription during isothermal amplification. |
| Detection Method | RT-LAMP with endpoint, turbidity, colorimetric or fluorescent readout, depending on the assay. |
| Sample Type | RNA targets used in RT-LAMP assays. |
| Performance Range / Specifications | 1,500 U; enzyme concentration 15 U/µL; supplied with a 10× freeze-dry-compatible isothermal buffer and 100 mM magnesium sulfate. |
| Specificity | Warm-start control reduces nonspecific low-temperature reverse transcription and improves assay specificity. |
| Reaction Conditions / Protocol | Combine with a compatible Bst polymerase, RT-LAMP primers, supplied buffer and magnesium sulfate. Activate and amplify at the temperature validated for the assay. |
| Components / Formulation | Glycerol-free reverse transcriptase at 15 U/µL; 10× freeze-dry-compatible isothermal amplification buffer; 100 mM magnesium sulfate. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 1,500 U. |
| Product Form | Glycerol-free reverse transcriptase kit for RT-LAMP formulation. |
| Quality Control | RT-LAMP efficiency, warm-start behavior, freeze–thaw stability and thermal storage stability are evaluated. |
| Key Features | Warm-start activation; strong reverse-transcription efficiency; glycerol-free formulation; RT-LAMP optimized; lyophilization compatible. |
| Concentration | 15 U/µL. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Reported performance remained stable after 60 freeze–thaw cycles, 4 weeks at 4°C and 7 days at 37°C. |
| Compatibility | Designed for RT-LAMP systems containing a compatible strand-displacing DNA polymerase. |
| Recommended Buffer System | 10× freeze-dry-compatible isothermal amplification buffer with separately supplied 100 mM MgSO4. |
| Application Notes / Precautions | No lyophilization bulking agent is included. Optimize magnesium concentration, enzyme ratio and drying conditions for the final assay. |
For research use only, not for clinical use.
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