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| Product Name | Glycerol-Free Warm-Start Reverse Transcriptase |
| Catalog No. | IEC-HMM-0105 |
| Description | A glycerol-free warm-start reverse transcriptase intended for custom and lyophilized one-step RT-qPCR reagent development. |
| Intended Use | Custom one-step RT-qPCR formulation, rapid reverse transcription and lyophilized molecular diagnostic reagent development. |
| Principle / Technology | Warm-start enzyme control limits low-temperature activity, while the glycerol-free formulation improves compatibility with freeze-drying workflows. |
| Detection Method | Used as a reverse-transcription component in one-step real-time PCR systems. |
| Sample Type | RNA templates in custom RT-qPCR assays. |
| Performance Range / Specifications | 10,000 U or 40,000 U; supports fast programs and balanced detection across high and low template levels. |
| Specificity | Warm-start control reduces nonspecific reverse transcription during reaction preparation. |
| Reaction Conditions / Protocol | Formulate with a compatible reaction buffer, hot-start DNA polymerase and assay-specific primers/probes. Optimize the enzyme input for the intended one-step RT-qPCR system. |
| Components / Formulation | Glycerol-free reverse transcriptase without added lyophilization excipients. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 10,000 U; 40,000 U. |
| Product Form | Glycerol-free reverse transcriptase enzyme solution. |
| Quality Control | Lyophilization performance, rapid-program compatibility, template-balance performance and stress stability are evaluated. |
| Key Features | Glycerol-free formulation; lyophilization compatible; warm-start activity; fast-program support; high storage stability. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Reported performance remained stable after 50 liquid-nitrogen freeze–thaw cycles, 4 weeks at 4°C and 30 days at 37°C. |
| Compatibility | Suitable for custom one-step RT-qPCR and lyophilized reagent development. |
| Application Notes / Precautions | The enzyme does not contain lyophilization bulking agents; select excipients and drying conditions for the final formulation. |
For research use only, not for clinical use.
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