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| Product Name | Glycerol-Free Lyophilization-Compatible Reverse Transcriptase |
| Catalog No. | IEC-HMM-0106 |
| Description | A glycerol-free reverse transcriptase designed for first-strand cDNA synthesis and custom lyophilized one-step RT-qPCR systems. |
| Intended Use | Reverse transcription, first-strand cDNA synthesis and custom one-step RT-qPCR reagent formulation. |
| Principle / Technology | The glycerol-free enzyme retains efficient reverse-transcription activity while improving compatibility with freeze-drying processes. |
| Detection Method | Used upstream of PCR or as the reverse-transcription component of a custom one-step RT-qPCR assay. |
| Sample Type | RNA templates, including viral and cellular RNA. |
| Performance Range / Specifications | 10,000 U or 40,000 U; demonstrated stable detection across mixed viral and cellular RNA inputs in a custom one-step RT-qPCR system. |
| Specificity | Efficient reverse transcription supports reliable target detection in custom one-step formulations. |
| Reaction Conditions / Protocol | Use in a compatible reverse-transcription buffer or custom one-step RT-qPCR formulation. Optimize enzyme and buffer concentrations for the intended assay. |
| Components / Formulation | Glycerol-free reverse transcriptase without added lyophilization excipients. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 10,000 U; 40,000 U. |
| Product Form | Glycerol-free reverse transcriptase enzyme solution. |
| Quality Control | Freeze-drying performance, thermal stability, batch consistency and one-step RT-qPCR activity are evaluated. |
| Key Features | Glycerol-free; suitable for freeze-drying; high stability; compatible with custom one-step RT-qPCR systems. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Reported Cq shifts remained within ±0.5 in freeze-drying, batch, and thermal stability evaluations. |
| Compatibility | Suitable for first-strand cDNA synthesis and custom liquid or lyophilized one-step RT-qPCR formulations. |
| Application Notes / Precautions | The final freeze-dried formulation requires separately selected excipients and an optimized drying cycle. |
For research use only, not for clinical use.
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