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| Product Name | Glycerol-Free Hot-Start Taq DNA Polymerase |
| Catalog No. | IEC-HMM-0109 |
| Description | A glycerol-free antibody-blocked hot-start Taq DNA polymerase for sensitive PCR and qPCR reagent development, including freeze-dried formulations. |
| Intended Use | Hot-start PCR, qPCR, low-copy target amplification and lyophilized molecular diagnostic reagent development. |
| Principle / Technology | A thermostable antibody blocks Taq polymerase at low temperatures and remains effective at 55°C. Heating at 95°C for at least 30 seconds releases full polymerase activity. |
| Detection Method | Endpoint PCR or fluorescent qPCR, depending on the assay formulation. |
| Sample Type | Genomic DNA, cDNA and other compatible DNA templates, including complex low-copy samples. |
| Performance Range / Specifications | 1,000 U or 5,000 U; fully activated after approximately 30 seconds at 95°C; compatible with varied PCR and qPCR buffer conditions. |
| Specificity | Low-temperature antibody blocking suppresses nonspecific amplification during setup and instrument ramping. |
| Reaction Conditions / Protocol | Formulate with a compatible PCR or qPCR buffer, dNTPs, primers and optional probe. Include an initial activation step of at least 95°C for 30 seconds. |
| Components / Formulation | Glycerol-free antibody-modified hot-start Taq DNA polymerase; reaction buffer is not included. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 1,000 U; 5,000 U. |
| Product Form | Glycerol-free hot-start DNA polymerase enzyme solution. |
| Quality Control | Hot-start blocking, heat activation, sensitivity, specificity and buffer compatibility are evaluated. |
| Key Features | Glycerol-free; lyophilization compatible; rapid heat activation; strong specificity; high sensitivity; broad PCR/qPCR compatibility. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for Taq-based PCR and qPCR formulations across a range of buffer pH and ionic-strength conditions. |
| Application Notes / Precautions | A reaction buffer is not supplied. Optimize magnesium, salt and stabilizer concentrations for the intended formulation. |
For research use only, not for clinical use.
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