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| Product Name | Glutamate Dehydrogenase GLDH Enzyme for Monoamine Oxidase Assay >=50 U/mg 50 kDa CAS 9029-12-3 |
| Catalog No. | IEC-HMM-0285 |
| Description | Glutamate dehydrogenase from Alta DiagnoTech reversibly catalyzes the reductive amination of alpha-ketoglutarate to L-glutamate using NADH and ammonium. It is applied in monoamine oxidase detection kits and ammonia/amino acid metabolism studies. |
| Intended Use | Enzyme raw material for monoamine oxidase assay kits and ammonia/glutamate assays. |
| Principle / Technology | Catalyzes the reversible reaction between alpha-ketoglutarate, ammonia and NADH to form L-glutamate and NAD. |
| Detection Method | NADH consumption measured at 340 nm. |
| Sample Type | Serum, plasma, research samples. |
| Performance Range / Specifications | Specific activity >=50 U/mg. Molecular weight approximately 50 kDa. Isoelectric point 5.92. |
| Components / Formulation | Glutamate dehydrogenase lyophilized powder. |
| Storage Conditions | -20°C. |
| Shelf Life | Stable for at least 12 months at -20°C. |
| Package Specifications | KU packaging units. |
| Product Form | White to pale yellow amorphous flocculent powder. |
| Purity | 99% |
| Activity / Unit Definition | >=50 U/mg |
| Molecular Weight | Approximately 50 kDa |
| Source / Origin | Microbial fermentation. |
| pH Range / Optimal pH | Optimal pH 7.0-8.0; stable between pH 5.0-10.0. |
| Shipping Conditions | Cold chain shipment. |
| Expiration Date / Stability | -20°C stable for one year. |
| Appearance | White to pale yellow amorphous flocculent powder. |
| CAS Number | 9029-12-3 |
| Isoelectric Point | 5.92 |
| Inhibitors | Not inhibited by sodium azide. |
| Optimal Temperature | Around 45°C. |
| Thermal Stability | Stable below 50°C. |
| Handling Precaution | Avoid repeated freeze-thaw; reconstitute in recommended buffer. |
| Michaelis Constant | 10.4×10^-3 mol/L (NH4Cl); 7.42×10^-3 mol/L (alpha-Ketoglutarate); 6.65×10^-4 mol/L (NADH) |
For research use only, not for clinical use.
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