Glucose Dehydrogenase NAD-Dependent Enzyme pI 5.3 32 kDa >=70 U/mg CAS 9028-53-9
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Glucose Dehydrogenase NAD-Dependent Enzyme pI 5.3 32 kDa >=70 U/mg CAS 9028-53-9

Cat.No: IEC-HMM-0279 Datasheet

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Product Name Glucose Dehydrogenase NAD-Dependent Enzyme pI 5.3 32 kDa >=70 U/mg CAS 9028-53-9
Catalog No. IEC-HMM-0279
Description Alta DiagnoTech glucose dehydrogenase is an NAD-dependent oxidoreductase that oxidizes glucose to gluconolactone. With high specific activity and excellent thermal stability, it is ideal for glucose determination in clinical chemistry and point-of-care devices.
Intended Use Raw enzyme for blood glucose assay reagents and glucose biosensors.
Principle / Technology Catalyzes the oxidation of beta-D-glucose using NAD as cofactor, producing NADH whose formation is monitored spectrophotometrically.
Detection Method NADH absorbance at 340 nm.
Sample Type Serum, plasma, whole blood.
Performance Range / Specifications Specific activity >=70 U/mg. Molecular weight 32 kDa. Isoelectric point 5.3.
Components / Formulation Glucose dehydrogenase lyophilized powder.
Storage Conditions -20°C.
Shelf Life Stable for at least 12 months at -20°C.
Package Specifications KU packaging units.
Product Form White amorphous powder.
Purity 99%
Activity / Unit Definition >=70 U/mg
Molecular Weight 32 kDa
Source / Origin Microbial fermentation.
pH Range / Optimal pH Optimal pH >9.0; stable between pH 5.0-9.5.
Shipping Conditions Cold chain shipment.
Expiration Date / Stability -20°C stable for one year.
Appearance White amorphous powder.
CAS Number 9028-53-9
Isoelectric Point 5.3
Inhibitors Not inhibited by sodium azide.
Optimal Temperature >60°C.
Thermal Stability Stable below 70°C.
Handling Precaution Keep dry; reconstitute in appropriate buffer immediately before use.
Michaelis Constant 0.8×10^-2 mol/L (Glucose); 1.5×10^-4 mol/L (NAD); 4.3×10^-5 mol/L (NADP)

For research use only, not for clinical use.

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