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| Product Name | Glucose Consumption Cell Viability Assay Kit (GOx-POD Coupled) |
| Catalog No. | CATR-HMM-0056 |
| Description | A colorimetric assay kit that quantifies cell viability and metabolic activity by measuring glucose consumption from the culture medium. Viable, metabolically active cells consume glucose from the medium; the residual glucose concentration is measured using a glucose oxidase-peroxidase (GOx-POD) coupled enzymatic reaction with a chromogenic substrate. This non-invasive method provides a direct measure of cellular metabolic rate, complementing ATP-based and reduction-based viability assays. |
| Intended Use | Measurement of cellular metabolic activity via glucose consumption; insulin response and glucose uptake studies; metabolic profiling of cancer cell lines; assessment of mitochondrial vs. glycolytic metabolic shift (Warburg effect); cell viability assessment in glucose-containing media. |
| Principle / Technology | Glucose oxidase oxidizes residual glucose to gluconic acid and H2O2; peroxidase couples H2O2 to oxidation of chromogenic substrate producing color proportional to glucose concentration; glucose consumption (viability indicator) is calculated as medium glucose minus residual glucose |
| Detection Method | Colorimetric; absorbance at 505 nm; endpoint assay |
| Sample Type | Cell culture supernatant from glucose-containing medium; requires glucose-free medium for experimental medium control |
| Sensitivity / LOD | Linear glucose detection range 0.02–2.0 mg/mL (0.11–11.1 mM); glucose consumption detection from 500 cells |
| Specificity | Highly specific for D-glucose; no cross-reactivity with galactose, fructose, mannose, or sucrose |
| Reaction Conditions / Protocol | Collect 2 µL culture supernatant; add 200 µL glucose detection reagent; incubate 10 minutes at 37 °C; read absorbance at 505 nm; calculate glucose consumption = initial glucose - residual glucose |
| Components / Formulation | Glucose oxidase (lyophilized), peroxidase (lyophilized), 4-aminoantipyrine (4-AAP) chromogen, phenol coupling reagent, glucose standard (1.0 mg/mL), assay buffer (phosphate, pH 7.0) |
| Storage Conditions | Store at 2–8 °C for liquid components; -20 °C for lyophilized enzymes; protect 4-AAP from light |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | 500 tests, 2,000 tests in 96-well format |
| Product Form | Lyophilized enzymes with liquid buffers and chromogens |
| Key Features | Non-invasive — uses spent medium, cells remain viable for downstream analysis; direct metabolic activity readout complementary to ATP and reducing potential assays; high-throughput compatible (96-well and 384-well); glucose standard included for calibration; wide linear range covers typical cell culture glucose consumption |
| Purity | Glucose oxidase specific activity >150 U/mg; peroxidase (HRP) RZ >3.0 |
| Concentration | As specified on product label; working concentrations optimized per protocol |
| Activity / Unit Definition | Signal-to-noise ratio and linearity verified on reference cell lines per lot |
| Molecular Weight | Varies by dye or reagent component as specified |
| Source / Origin | Synthetic dyes and tetrazolium compounds; recombinant enzymes where applicable |
| pH Range / Optimal pH | pH 7.2–7.4 for cell-based assays |
| Shipping Conditions | Cold pack 2–8 °C with lyophilized enzymes at -20 °C |
| Expiration Date / Stability | 12 months under recommended storage; reconstituted glucose standard stable at 4 °C for 1 month; working reagent prepare fresh daily |
| Regulatory / Compliance | For laboratory and research use only; RUO; manufactured under ISO 9001; components not classified as dangerous goods |
| Compatibility | Compatible with DMEM, RPMI-1640, MEM, and most glucose-containing cell culture media; phenol red does not interfere at standard concentrations; reducing agents (DTT, β-mercaptoethanol, ascorbic acid) interfere with peroxidase reaction — avoid or remove before assay |
| Recommended Buffer System | PBS or phenol red-free complete medium as specified in protocol |
| Application Notes / Precautions | For accurate glucose consumption calculation, measure glucose in fresh medium (time zero) and in conditioned medium at endpoint. Include medium-only incubated control (no cells) to account for spontaneous glucose degradation. For insulin response studies, pre-incubate cells in low-glucose (1 g/L) or glucose-free medium for 2–4 hours before assay. Normalize glucose consumption to cell number determined by parallel viability assay. |
| Batch-to-Batch Consistency | Signal linearity R² ≥0.99 with reference cell number standard curve per lot |
For research use only, not for clinical use.
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