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| Product Name | Full-Length cDNA Reverse Transcriptase |
| Catalog No. | IEC-HMM-0116 |
| Description | An engineered RNase H-deficient reverse transcriptase for high-temperature synthesis of full-length first-strand cDNA from long or structured RNA templates. |
| Intended Use | First-strand cDNA synthesis, two-step RT-qPCR, RT-PCR and full-length transcript analysis. |
| Principle / Technology | An engineered M-MLV-derived enzyme provides RNA-dependent and DNA-dependent DNA polymerase activities together with terminal transferase activity and reduced RNase H activity. |
| Detection Method | Downstream PCR, qPCR, cloning or sequencing of synthesized cDNA. |
| Sample Type | Purified RNA templates, including long or structured transcripts. |
| Performance Range / Specifications | cDNA synthesis up to approximately 20 kb; reverse-transcription temperatures up to 65°C; 2,000 U or 10,000 U. |
| Sensitivity / LOD | High processivity supports efficient synthesis across long RNA templates. |
| Specificity | Elevated-temperature reverse transcription helps resolve RNA secondary structure and improves primer-specific cDNA synthesis. |
| Reaction Conditions / Protocol | Combine RNA, primer, dNTPs, compatible reverse-transcription buffer and enzyme, then incubate at a validated temperature up to 65°C before heat inactivation or downstream use. |
| Components / Formulation | Full-length cDNA reverse transcriptase enzyme solution. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 2,000 U; 10,000 U. |
| Product Form | Purified reverse transcriptase solution. |
| Key Features | cDNA products up to 20 kb; operation up to 65°C; high processivity; tolerance to guanidine hydrochloride, humic acid, heme, isopropanol and ethanol. |
| Source / Origin | Engineered from M-MLV RNase H-deficient reverse transcriptase. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for first-strand cDNA synthesis and two-step RT-qPCR or RT-PCR workflows. |
| Application Notes / Precautions | Use RNase-free materials and optimize primer type, reaction temperature and RNA input for transcript length and structure. |
For research use only, not for clinical use.
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