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| Product Name | Fast Restriction Enzyme XhoI (Recognition Site: C/TCGAG) |
| Catalog No. | IVDRM-0095 |
| Description | This fast restriction enzyme is a recombinant enzyme produced through genetic engineering. It precisely cleaves DNA within 5-15 minutes and is ideal for rapid digestion of plasmid DNA, PCR products, and genomic DNA. All enzymes in this fast digestion series share a single universal buffer, which greatly simplifies reaction setup. In addition, the enzyme offers ample activity redundancy, making it easy to digest excess substrate or difficult templates. |
| Intended Use | For rapid digestion of plasmid DNA, PCR products, and genomic DNA in molecular cloning and related molecular biology workflows. |
| Principle / Technology | Recombinant type II restriction endonuclease produced by genetic engineering. It recognizes a specific double-stranded DNA sequence and completes precise cleavage within 5-15 minutes. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Recognition site: 5'-C↓TCGAG-3' / 3'-GAGCT↑C-5' Enzyme activity: 20 U/μL Recommended reaction conditions: 1× universal fast digestion buffer; optimal incubation at 37°C Heat inactivation: 80°C for 20 min Isoschizomers: PaeR7I, TliI, BssHI, Sfr274I, SlaI, StrI Methylation sensitivity: CpG methylation (complete overlap) blocks cleavage |
| Specificity | Specifically recognizes and cleaves the sequence 5'-C↓TCGAG-3'. Cleavage is blocked when CpG methylation fully overlaps the recognition site. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; optimal incubation at 37°C for 5-15 minutes. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 500 T |
| Concentration | 20 U/μL |
| Activity / Unit Definition | 20 U/μL |
| Source / Origin | Recombinant enzyme produced by genetic engineering. |
| Expiration Date / Stability | Valid for 2 years when stored at -25 to -15°C. |
| Compatibility | Isoschizomers: PaeR7I, TliI, BssHI, Sfr274I, SlaI, StrI |
| Recommended Buffer System | 1× universal fast digestion buffer (shared by all enzymes in the fast restriction enzyme series, simplifying reaction setup). |
| Application Notes / Precautions | The enzyme has high activity redundancy, allowing easy digestion of excess substrate or difficult templates. Note that CpG methylation fully overlapping the recognition site blocks cleavage. |
| Recognition Site | 5'-C↓TCGAG-3' 3'-GAGCT↑C-5' |
| Heat Inactivation | Incubate at 80°C for 20 min. |
| Methylation Sensitivity | CpG: complete overlap with the sequence; cleavage blocked. |
For research use only, not for clinical use.
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