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| Product Name | Fast Restriction Enzyme XcmI (Recognition Site: 5'-CCA(N)5↓(N)4TGG-3') |
| Catalog No. | IVDRM-0097 |
| Description | Fast Restriction Enzyme XcmI is a genetically engineered recombinant restriction endonuclease that delivers precise DNA cleavage in as little as 5-15 minutes. It is ideal for the rapid digestion of plasmid DNA, PCR products, and genomic DNA. All enzymes in this fast digestion series share a single universal reaction buffer, greatly simplifying reaction setup, and exhibit generous activity redundancy, enabling effortless digestion of excess substrate or difficult templates. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products, and genomic DNA for molecular cloning workflows. |
| Principle / Technology | A recombinant restriction endonuclease produced by genetic engineering that recognizes a specific interrupted palindromic sequence and cleaves double-stranded DNA at defined positions within minutes at 37°C. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA. |
| Performance Range / Specifications | Concentration: 5 U/μL Complete digestion in 5-15 minutes High activity redundancy for excess substrate or difficult templates |
| Specificity | Recognizes and cleaves the sequence 5'-CCA(N)5↓(N)4TGG-3' / 3'-GGT(N)4↑(N)5ACC-5'. |
| Reaction Conditions / Protocol | Digest in 1× universal fast digestion buffer; optimal incubation at 37°C for 5-15 minutes. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years when stored at -25°C to -15°C. |
| Package Specifications | 60 T |
| Concentration | 5 U/μL |
| Source / Origin | Recombinant enzyme produced by genetic engineering. |
| Expiration Date / Stability | Stable for 2 years at -25°C to -15°C. |
| Compatibility | No known isoschizomers. Shares a universal fast digestion buffer with other enzymes in the same fast digestion series, enabling convenient double or multiple digestions. |
| Recommended Buffer System | 1× universal fast digestion buffer. |
| Application Notes / Precautions | The shared universal buffer simplifies reaction setup when multiple enzymes of the series are used together. The enzyme provides sufficient activity redundancy to handle substrate excess or difficult templates. |
| Recognition Site | 5'-CCA(N)5↓(N)4TGG-3' 3'-GGT(N)4↑(N)5ACC-5' |
| Heat Inactivation | Incubate at 80°C for 20 minutes. |
| Methylation Sensitivity | Not affected by Dam, Dcm, CpG, EcoKI, or EcoBI methylation. |
For research use only, not for clinical use.
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