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| Product Name | Fast Restriction Enzyme XbaI, High Concentration (100 U/μL) |
| Catalog No. | IVDRM-0096 |
| Description | This fast restriction enzyme is a recombinant enzyme produced through genetic engineering. It precisely cleaves DNA within 5-15 minutes and is ideal for rapid digestion of plasmid DNA, PCR products, and genomic DNA. All enzymes in this fast digestion series share a single universal buffer, which greatly simplifies reaction setup. In addition, the enzyme offers ample activity redundancy, making it easy to digest excess substrate or difficult templates. This formulation is supplied at a high concentration of 100 U/μL for demanding or high-throughput applications. |
| Intended Use | For rapid digestion of plasmid DNA, PCR products, and genomic DNA in molecular cloning and related molecular biology workflows. |
| Principle / Technology | Recombinant type II restriction endonuclease produced by genetic engineering. It recognizes a specific double-stranded DNA sequence and completes precise cleavage within 5-15 minutes. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Recognition site: 5'-T↓CTAGA-3' / 3'-AGATC↑T-5' Enzyme activity: 100 U/μL Recommended reaction conditions: 1× universal fast digestion buffer; optimal incubation at 37°C Heat inactivation: 80°C for 20 min Isoschizomer: none Methylation sensitivity: Dam methylation (overlapping sequence) blocks cleavage |
| Specificity | Specifically recognizes and cleaves the sequence 5'-T↓CTAGA-3'. Cleavage is blocked when the site overlaps with Dam methylation. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; optimal incubation at 37°C for 5-15 minutes. |
| Storage Conditions | Store at -20°C. |
| Shelf Life | 2 years when stored at -20°C. |
| Package Specifications | 10,000 U |
| Concentration | 100 U/μL |
| Activity / Unit Definition | 100 U/μL |
| Source / Origin | Recombinant enzyme produced by genetic engineering. |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for 2 years when stored at -20°C. |
| Recommended Buffer System | 1× universal fast digestion buffer (shared by all enzymes in the fast restriction enzyme series, simplifying reaction setup). |
| Application Notes / Precautions | The enzyme has high activity redundancy, allowing easy digestion of excess substrate or difficult templates. Note that cleavage is blocked when the recognition site overlaps with Dam methylation. |
| Recognition Site | 5'-T↓CTAGA-3' 3'-AGATC↑T-5' |
| Heat Inactivation | Incubate at 80°C for 20 min. |
| Methylation Sensitivity | Dam: the sequence may overlap, blocking cleavage. |
For research use only, not for clinical use.
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