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| Product Name | Fast Restriction Enzyme Tsp45I (Recognition Site: GTSAC) |
| Catalog No. | IVDRM-0091 |
| Description | Fast Restriction Enzyme Tsp45I is a recombinant restriction endonuclease engineered for rapid, highly precise DNA cleavage, completing digestion within 5-15 minutes. It is ideal for fast cutting of plasmid DNA, PCR products, and genomic DNA. All fast restriction enzymes in this series share one universal digestion buffer, which greatly simplifies reaction setup, and their generous activity reserve allows effortless digestion of excess substrate or difficult templates. |
| Intended Use | For rapid, precise digestion of plasmid DNA, PCR products, and genomic DNA in molecular cloning and other molecular biology workflows. For research use. |
| Principle / Technology | Recombinant fast restriction endonuclease produced by genetic engineering. It recognizes a specific double-stranded DNA sequence and cleaves both strands at defined positions, completing accurate digestion within 5-15 minutes. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Complete digestion within 5-15 minutes; high activity reserve readily handles excess substrate or difficult templates. |
| Specificity | Recognizes the degenerate sequence 5'-GTSAC-3' (S = G or C) and cleaves upstream of the site, generating 5' overhangs. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; incubate at 65°C. Digestion is typically complete within 5-15 minutes. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years when stored at -25°C to -15°C. |
| Package Specifications | 25 T |
| Concentration | 10 U/μL |
| Activity / Unit Definition | 10 U/μL |
| Source / Origin | Recombinant expression (genetically engineered). |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Compatibility | Isoschizomers: NmuCI, TseFI |
| Recommended Buffer System | All fast restriction enzymes in this series share a single universal digestion buffer, which simplifies reaction setup. |
| Application Notes / Precautions | Optimal incubation temperature is 65°C. Tsp45I cannot be heat-inactivated; remove the enzyme by phenol-chloroform extraction or column purification after digestion. EcoBI methylation blocks cleavage; Dam, Dcm, CpG, and EcoKI methylation have no effect. |
| Recognition Site | 5'-↓GTSAC-3' 3'-CASTG↑-5' (S = G or C) |
| Heat Inactivation | Cannot be heat-inactivated; use phenol-chloroform extraction or column purification. |
| Methylation Sensitivity | EcoBI methylation: cleavage blocked. Dam, Dcm, CpG, EcoKI methylation: no effect. |
For research use only, not for clinical use.
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