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| Product Name | Fast Restriction Enzyme SapI (Recognition Site: GCTCTTC(N)1) |
| Catalog No. | IVDRM-0089 |
| Description | Fast Restriction Enzyme SapI is a recombinant Type IIS restriction endonuclease engineered for rapid, highly precise DNA cleavage, completing digestion within 5-15 minutes. It is ideal for fast cutting of plasmid DNA, PCR products, and genomic DNA. All fast restriction enzymes in this series share one universal digestion buffer, which greatly simplifies reaction setup, and their generous activity reserve allows effortless digestion of excess substrate or difficult templates. |
| Intended Use | For rapid, precise digestion of plasmid DNA, PCR products, and genomic DNA in molecular cloning and other molecular biology workflows. For research use. |
| Principle / Technology | Recombinant fast restriction endonuclease produced by genetic engineering. It recognizes a specific double-stranded DNA sequence and cleaves both strands at defined positions, completing accurate digestion within 5-15 minutes. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Complete digestion within 5-15 minutes; high activity reserve readily handles excess substrate or difficult templates. |
| Specificity | Type IIS restriction enzyme: recognizes the asymmetric sequence 5'-GCTCTTC-3' and cleaves outside the recognition site, 1 nt downstream on the top strand and 4 nt on the bottom strand, generating defined overhangs. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; incubate at 37°C. Digestion is typically complete within 5-15 minutes. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years when stored at -25°C to -15°C. |
| Package Specifications | 50 T |
| Concentration | 10 U/μL |
| Activity / Unit Definition | 10 U/μL |
| Source / Origin | Recombinant expression (genetically engineered). |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Compatibility | Isoschizomers: BspQI, LguI, PciSI |
| Recommended Buffer System | All fast restriction enzymes in this series share a single universal digestion buffer, which simplifies reaction setup. |
| Application Notes / Precautions | SapI is a Type IIS restriction enzyme that cleaves outside its recognition sequence, making it well suited for seamless cloning strategies such as Golden Gate assembly. |
| Recognition Site | 5'-GCTCTTC(N)1↓-3' 3'-CGAGAAG(N)4↑-5' |
| Heat Inactivation | 80°C for 20 min. |
For research use only, not for clinical use.
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