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| Product Name | Fast Restriction Enzyme PstI (Recognition Site: 5'-CTGCA↓G-3') |
| Catalog No. | IVDRM-0067 |
| Description | A genetically engineered, recombinant fast restriction enzyme that completes precise DNA digestion within 5–15 minutes, suitable for rapid cleavage of plasmid DNA, PCR products and genomic DNA. All fast restriction enzymes in this series share a single universal digestion buffer, greatly simplifying reaction setup. The enzyme also offers a generous activity margin, allowing it to handle excess substrate or difficult templates with ease. |
| Intended Use | For rapid digestion of plasmid DNA, PCR products and genomic DNA in molecular cloning workflows. For research use. |
| Principle / Technology | Recombinant type II restriction endonuclease produced by genetic engineering; it recognizes a specific double-stranded DNA sequence and cleaves it rapidly (within 5–15 minutes) in a universal fast digestion buffer. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Completes precise DNA digestion within 5–15 minutes High activity margin; easily handles excess substrate or difficult templates Enzyme activity: 20 U/μL Optimal incubation at 37°C |
| Specificity | Recognizes and cleaves 5'-CTGCA↓G-3' / 3'-G↑ACGTC-5'. Not affected by Dam, Dcm, CpG, EcoKI or EcoBI methylation. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; incubate at 37°C (optimal temperature). Digestion is typically complete within 5–15 minutes. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | Stable for 2 years when stored as recommended. |
| Package Specifications | 500 T |
| Concentration | 20 U/μL |
| Source / Origin | Recombinant enzyme produced by genetic engineering |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Compatibility | Isoschizomer: BspMAI |
| Recommended Buffer System | All fast restriction enzymes in the series share one universal fast digestion buffer, so single, double and multiple digests can be performed in the same reaction system. |
| Application Notes / Precautions | This enzyme cannot be heat inactivated; use phenol-chloroform extraction or column purification to stop the reaction. |
| Recognition Site | 5'-CTGCA↓G-3' 3'-G↑ACGTC-5' |
| Heat Inactivation | Cannot be heat inactivated; use phenol-chloroform extraction or column purification. |
| Methylation Sensitivity | Dam, Dcm, CpG, EcoKI, EcoBI: no effect |
For research use only, not for clinical use.
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