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| Product Name | Fast Restriction Enzyme PacI (Recognition Site: 5'-TTAAT↓TAA-3') |
| Catalog No. | IVDRM-0068 |
| Description | A genetically engineered, recombinant fast restriction enzyme that completes precise DNA digestion within 5–15 minutes, suitable for rapid cleavage of plasmid DNA, PCR products and genomic DNA. All fast restriction enzymes in this series share a single universal digestion buffer, greatly simplifying reaction setup. The enzyme also offers a generous activity margin, allowing it to handle excess substrate or difficult templates with ease. |
| Intended Use | For rapid digestion of plasmid DNA, PCR products and genomic DNA in molecular cloning workflows. For research use. |
| Principle / Technology | Recombinant type II restriction endonuclease produced by genetic engineering; it recognizes a specific double-stranded DNA sequence and cleaves it rapidly (within 5–15 minutes) in a universal fast digestion buffer. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Completes precise DNA digestion within 5–15 minutes High activity margin; easily handles excess substrate or difficult templates Enzyme activity: 10 U/μL Optimal incubation at 37°C |
| Specificity | Recognizes and cleaves 5'-TTAAT↓TAA-3' / 3'-AAT↑TAATT-5'. EcoKI methylation sites may overlap the recognition sequence and cleavage may be affected. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; incubate at 37°C (optimal temperature). Digestion is typically complete within 5–15 minutes. |
| Storage Conditions | Store at -20°C. |
| Shelf Life | Stable for 2 years when stored as recommended. |
| Package Specifications | 25 T |
| Concentration | 10 U/μL |
| Source / Origin | Recombinant enzyme produced by genetic engineering |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for 2 years when stored at -20°C. |
| Recommended Buffer System | All fast restriction enzymes in the series share one universal fast digestion buffer, so single, double and multiple digests can be performed in the same reaction system. |
| Application Notes / Precautions | Heat inactivate at 80°C for 20 min when enzyme removal is required. |
| Recognition Site | 5'-TTAAT↓TAA-3' 3'-AAT↑TAATT-5' |
| Heat Inactivation | Incubate at 80°C for 20 min. |
| Methylation Sensitivity | EcoKI: the sequence may overlap, and cleavage may be affected |
For research use only, not for clinical use.
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