Enhanced Murine RNase Inhibitor
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Enhanced Murine RNase Inhibitor

Cat.No: IEC-HMM-0103 Datasheet

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Product Details Related Products
Product Name Enhanced Murine RNase Inhibitor
Catalog No. IEC-HMM-0103
Description An enhanced recombinant murine RNase inhibitor with strong RNase A protection, high heat tolerance and improved oxidative stability.
Intended Use First-strand cDNA synthesis; RT-PCR and RT-qPCR; in vitro transcription or translation; RNA purification; RNase protection assays.
Principle / Technology The inhibitor binds and suppresses RNase activity, protecting RNA integrity during enzymatic processing and analytical workflows.
Detection Method Used as a protective reagent in RNA workflows rather than as a standalone detection system.
Sample Type RNA-containing reaction mixtures and RNA purification samples at risk of RNase contamination.
Performance Range / Specifications 2,000 U, 10,000 U or 20,000 U; reported protection threshold against RNase A reached 10 ng.
Specificity Inhibits RNase activity while having minimal effect on one-step RT-qPCR sensitivity and fluorescence plateau values.
Reaction Conditions / Protocol Add to reverse-transcription, RT-PCR, RT-qPCR, in vitro transcription/translation or RNA-purification reactions at an application-appropriate unit amount.
Storage Conditions Store at −30 to −15°C.
Package Specifications 2,000 U; 10,000 U; 20,000 U.
Product Form Recombinant murine RNase inhibitor solution.
Quality Control RNase A protection, heat tolerance, oxidative stability and compatibility with one-step RT-qPCR are evaluated.
Key Features Protection against up to 10 ng RNase A in the reported evaluation; high heat tolerance; enhanced oxidative stability; minimal effect on RT-qPCR sensitivity.
Shipping Conditions Ship at or below 0°C.
Compatibility Suitable for reverse transcription, RT-PCR, RT-qPCR, RNA purification, in vitro transcription/translation and RNase protection assays.

For research use only, not for clinical use.

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