| Product Name | EdU Universal Cell Proliferation Assay Reagent (Green Fluorescence) |
| Catalog No. | CATR-HMM-0005 |
| Description | Cell proliferation assays are fundamental experimental tools for assessing cell health, genetic toxicity, and the efficacy of anticancer drugs. The most precise method for detecting cell proliferation is the BrdU assay, and EdU assay reagents represent a revolutionary breakthrough in this field. EdU (5-ethynyl-2'-deoxyuridine) is a pyrimidine analog that integrates into the DNA double helix during DNA synthesis. The detection is based on the “click” reaction, a covalent reaction catalyzed by copper between azide compounds and alkyne groups to form covalent bonds. The EdU labeling method using the click reaction is rapid, effective, and easy to use. It only requires formaldehyde fixation and Triton X-100 permeabilization to allow the detection reagent to enter the cells, and a small amount of azide dye is sufficient to effectively label the integrated EdU. In contrast, the BrdU method requires DNA denaturation (such as acid denaturation, heat denaturation, or DNase digestion) to expose the BrdU, thereby facilitating BrdU antibody binding. |
| Application | This product can be used in research on cell proliferation, differentiation, growth and development, DNA damage repair, and virus replication. |
| Applicable Instruments | Fluorescence microscope, flow cytometer |
| Materials to Bring | Centrifuge tube; 0.85% NaCl; 0.7 mL isopropanol |
| Storage | Store at -20°C away from light. |
| Features | Simple and efficient: No antigen-antibody reaction is required. This detection method, based on small molecule chemical reactions, is simple and efficient, with reactions taking only a few minutes. High sensitivity: No antibodies are required. The detection dye is only 1/500th of the BrdU antibody and diffuses easily, enabling accurate detection of even a single proliferating cell. Fast and time-saving: No overnight incubation is required, eliminating the complex and cumbersome steps of antigen-antibody reactions. The entire detection cycle can be completed in just 5 hours. |
| Notes | 1. Before use, centrifuge the product briefly to the bottom of the tube, then proceed with subsequent experiments. 2. When using EdU (10 mM) for the first time, it is recommended to aliquot it according to experimental needs and store at -20°C. 3. When preparing the Click-iT EdU buffer additive solution for the first time, it is recommended to aliquot it according to experimental needs and store at -20°C. If white particles precipitate after dissolution, invert the solution multiple times until completely dissolved before use. If the solution turns brown, it indicates that the active components of the solution have degraded and should be discarded. 4. Cells not treated with EdU can be stained with YF Dye Azide as negative control samples to determine appropriate detection conditions. This setting is particularly important for flow cytometry detection, as it helps clarify the intensity of negative signals to distinguish between negative and positive signals. 5. This product is not recommended for simultaneous detection with TUNEL kits. This is because the EdU structure contains -OH groups, which may interfere with the TUNEL reaction process. 6. This product is a copper ion-catalyzed click reaction, which may cause quenching of certain fluorescent proteins or dyes. It is recommended to perform the reaction and detection after the click reaction is complete. Currently, the affected dyes include PE and PE tandem dyes. 7. Copper ions can affect the fluorescence of fluorescent proteins such as GFP, RFP, and mCherry. Therefore, this product is not suitable for cell detection with GFP, RFP, mCherry, or other fluorescent proteins. 8. Since copper ions can disrupt actin structure and affect phalloidin detection, phalloidin is incompatible with this product. We recommend using Tubulin-Tracker Red for cell microtubule detection. 9. Store opened components according to the instructions in the manual. 10. Fluorescent dyes are susceptible to quenching. Please avoid exposure to light during experiments to slow down fluorescence quenching. |
For research use only, not for clinical use.
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