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| Product Name | E. coli Uracil-DNA Glycosylase |
| Catalog No. | IEC-HMM-0099 |
| Description | An E. coli uracil-DNA glycosylase for selective removal of uracil bases from single- or double-stranded DNA. |
| Intended Use | Control of carryover contamination in PCR and qPCR; removal of uracil from single- or double-stranded DNA. |
| Principle / Technology | The enzyme releases uracil from uracil-containing DNA, creating abasic sites that prevent effective amplification of dU-containing carryover products. |
| Detection Method | Used as a pretreatment enzyme within PCR or qPCR workflows. |
| Sample Type | Single- or double-stranded DNA containing uracil, including dU-containing PCR products. |
| Performance Range / Specifications | Available in 500 U and 5,000 U formats. The enzyme acts on uracil-containing single- and double-stranded DNA but does not efficiently remove uracil from oligonucleotides of six bases or fewer. |
| Specificity | Selective for uracil residues in DNA. |
| Reaction Conditions / Protocol | Add the enzyme to a dUTP-based PCR or qPCR contamination-control workflow and use a protocol appropriate for a non-thermolabile UDG. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 500 U; 5,000 U. |
| Product Form | Uracil-DNA glycosylase enzyme solution. |
| Quality Control | Enzymatic activity and contaminating nuclease levels are controlled. |
| Key Features | Effective dU-DNA removal; compatible with single- and double-stranded DNA; suitable for PCR carryover prevention; quality-controlled enzyme preparation. |
| Source / Origin | E. coli uracil-DNA glycosylase. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Designed for dUTP-containing PCR and qPCR systems requiring enzymatic carryover control. |
| Application Notes / Precautions | This enzyme is not heat-labile. Use an assay program that prevents residual UDG activity from affecting newly synthesized uracil-containing products. |
For research use only, not for clinical use.
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