D-Lactate Dehydrogenase in 10-1,000 kU Formats for ALT/AST Reagents

Name: D-Lactate Dehydrogenase in 10-1,000 kU Formats for ALT/AST Reagents
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Cat.No: IEC-HMM-0182 Datasheet

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Product Name	D-Lactate Dehydrogenase in 10-1,000 kU Formats for ALT/AST Reagents
Catalog No.	IEC-HMM-0182
Description	Alta DiagnoTech D-lactate dehydrogenase is a white amorphous lyophilized enzyme powder with low oxidase and dehydrogenase contaminants for aminotransferase reagent systems.
Intended Use	Enzyme raw material for research and bulk formulation of aspartate aminotransferase and alanine aminotransferase reagents.
Performance Range / Specifications	Specific activity >=420.0 U/mg dry powder; purity >=90.0% by SDS-PAGE; NADH/NADPH oxidase <=0.01%; malate dehydrogenase <=0.005%; glutamate dehydrogenase <=0.003%; pyruvate kinase <=0.03%.
Storage Conditions	Store at -25 to -15 degrees C
Shelf Life	Maintains at least 90% activity for 12 months at -25 to -15 degrees C
Package Specifications	10 kU, 100 kU, 500 kU or 1,000 kU
Product Form	White amorphous lyophilized powder
Purity	>=90.0% by SDS-PAGE
Activity / Unit Definition	One unit is the amount of enzyme that oxidizes 1 umol NADH per minute under the stated conditions.
Molecular Weight	45 kDa by SDS-PAGE
Source / Origin	Microbial source
pH Range / Optimal pH	Optimum pH 6.5-7.0; stable at pH 5.5-10.0 for 16 hours at 25 degrees C
Appearance	White amorphous lyophilized powder
Alias	D-Lactate Dehydrogenase
Product Type	D-lactate dehydrogenase enzyme raw material
Function / Efficacy	Oxidize NADH in coupled ALT and AST reagent systems.
Abbreviation	D-LDH
EC Number	EC 1.1.1.28
Specific Activity	>=420.0 U/mg dry powder
Isoelectric Point	4.5
Inhibitors	Ag+ and Hg2+
Optimal Temperature	45 degrees C
Thermal Stability	Stable below 50 degrees C at pH 7.0 for 30 minutes
Michaelis Constant	2.2 x 10^-6 M for pyruvate
Stabilizer	BSA

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For research use only, not for clinical use.