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| Product Name | Creatininase (CAH) |
| Catalog No. | IEC-HMM-0128 |
| Description | Alta DiagnoTech Creatininase (CAH) is a recombinant enzyme supplied as white lyophilized powder. The enzyme has a molecular weight of 47 kDa (SDS-PAGE) by SDS-PAGE. |
| Intended Use | For enzymatic creatinine determination in clinical analysis when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase, and formaldehyde dehydrogenase. |
| Principle / Technology | One unit converts 1 µmol of creatinine to creatine per minute at pH 7.5 and 37°C. |
| Performance Range / Specifications | EC 3.5.2.10; CAS 9025-13-2; Specific activity: ≥500 U/mg protein |
| Reaction Conditions / Protocol | Optimum pH: 6.5-7.5; Optimum temperature: 70°C; pH stability: pH 8.5-9.0 at 25°C for 16 hours; Thermal stability: Below 70°C at pH 7.4 for 30 minutes; Inhibitors: Ag+, Hg2+, N-bromosuccinimide, and EDTA |
| Components / Formulation | White lyophilized powder |
| Storage Conditions | Store at or below -20°C. |
| Shelf Life | 18 months at -20°C. |
| Product Form | White lyophilized powder |
| Key Features | White lyophilized powder; Molecular weight: 47 kDa (SDS-PAGE); EC 3.5.2.10; Specific activity: ≥500 U/mg protein; Optimum pH: 6.5-7.5 |
| Activity / Unit Definition | Specific activity: ≥500 U/mg protein; Unit definition: One unit converts 1 µmol of creatinine to creatine per minute at pH 7.5 and 37°C. |
| Molecular Weight | 47 kDa (SDS-PAGE) |
| Source / Origin | Recombinant |
| pH Range / Optimal pH | 6.5-7.5 |
| Expiration Date / Stability | pH 8.5-9.0 at 25°C for 16 hours; Below 70°C at pH 7.4 for 30 minutes |
| Compatibility | Creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase, and formaldehyde dehydrogenase |
| Alternative Names / Synonyms | Creatininase (CAH); CAH |
For research use only, not for clinical use.
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