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| Product Name | Creatine Amidinohydrolase (CRH) |
| Catalog No. | IEC-HMM-0129 |
| Description | Alta DiagnoTech Creatine Amidinohydrolase (CRH) is a recombinant enzyme supplied as white lyophilized powder. The enzyme has a molecular weight of 30 kDa (SDS-PAGE) by SDS-PAGE. |
| Intended Use | For enzymatic determination of creatine and creatinine in clinical analysis when coupled with creatinine amidohydrolase, sarcosine dehydrogenase or sarcosine oxidase, and formaldehyde dehydrogenase. |
| Principle / Technology | One unit produces 1 µmol of sarcosine per minute at pH 7.5 and 37°C. |
| Performance Range / Specifications | EC 3.5.3.3; CAS 37340-58-2; Specific activity: ≥20 U/mg protein |
| Reaction Conditions / Protocol | Optimum pH: 7.0-9.0; Optimum temperature: 50°C; pH stability: pH 5.0-10.5 at 25°C for 16 hours; Thermal stability: Below 50°C at pH 7.5 for 30 minutes; Inhibitors: Ag+, Hg2+, and Cu2+ |
| Components / Formulation | White lyophilized powder |
| Storage Conditions | Store at or below -20°C. |
| Shelf Life | 18 months at -20°C. |
| Product Form | White lyophilized powder |
| Key Features | White lyophilized powder; Molecular weight: 30 kDa (SDS-PAGE); EC 3.5.3.3; Specific activity: ≥20 U/mg protein; Optimum pH: 7.0-9.0 |
| Activity / Unit Definition | Specific activity: ≥20 U/mg protein; Unit definition: One unit produces 1 µmol of sarcosine per minute at pH 7.5 and 37°C. |
| Molecular Weight | 30 kDa (SDS-PAGE) |
| Source / Origin | Recombinant |
| pH Range / Optimal pH | 7.0-9.0 |
| Expiration Date / Stability | pH 5.0-10.5 at 25°C for 16 hours; Below 50°C at pH 7.5 for 30 minutes |
| Compatibility | Creatinine amidohydrolase, sarcosine dehydrogenase or sarcosine oxidase, and formaldehyde dehydrogenase |
| Alternative Names / Synonyms | Creatine Amidinohydrolase (CRH); CRH |
For research use only, not for clinical use.
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