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| Product Name | Creatinase Enzyme for Creatinine Assay Development >=5 U/mg 47 kDa Isoelectric Point 6.2 CAS 37340-58-2 |
| Catalog No. | IEC-HMM-0275 |
| Description | High-purity creatinase supplied by Alta DiagnoTech as a lyophilized powder. This enzyme catalyzes the hydrolysis of creatine to sarcosine and urea, serving as a key reagent in enzymatic creatinine test kits for clinical chemistry analyzers. |
| Intended Use | Raw material for enzymatic creatinine assay kits and related in vitro diagnostic reagent manufacturing. |
| Principle / Technology | Creatinase hydrolyzes creatine to sarcosine and urea; when coupled with sarcosine oxidase and a chromogenic system, the rate of color formation correlates with creatinine concentration. |
| Detection Method | UV-visible spectrophotometry in coupled enzyme systems. |
| Sample Type | Serum, plasma, urine. |
| Performance Range / Specifications | Specific activity >=5 U/mg. Molecular weight approximately 47 kDa. Isoelectric point 6.2. |
| Components / Formulation | Creatinase lyophilized powder. |
| Storage Conditions | -20°C. |
| Shelf Life | Stable for at least 12 months at -20°C. |
| Package Specifications | KU packaging units; also available in 1 g bottle upon request. |
| Product Form | White amorphous powder. |
| Purity | 99% |
| Activity / Unit Definition | >=5 U/mg |
| Molecular Weight | 47 kDa |
| Source / Origin | Microbial fermentation. |
| pH Range / Optimal pH | Optimal pH 6.0-7.5; stable between pH 4.0-10.0 at 37°C for 24 hours. |
| Shipping Conditions | Cold chain shipment recommended. |
| Expiration Date / Stability | -20°C stable for one year. |
| Appearance | White amorphous powder. |
| CAS Number | 37340-58-2 |
| Isoelectric Point | 6.2 |
| Inhibitors | Not inhibited by sodium azide. |
| Optimal Temperature | 40-50°C. |
| Thermal Stability | Stable below 50°C for 15 minutes. |
| Handling Precaution | Avoid repeated freeze-thaw cycles. Reconstitute immediately before use. |
For research use only, not for clinical use.
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