- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Coenzyme A, Trilithium Salt, >=95% Purity, HPLC Grade |
| Catalog No. | IEC-HMM-0079 |
| Description | High-purity coenzyme A (CoA) as the trilithium salt, suitable for biochemical assays, enzymatic synthesis, and metabolic research. Coenzyme A is the essential acyl group carrier and cofactor involved in over 4% of all known enzymatic reactions, including the tricarboxylic acid cycle, fatty acid synthesis and beta-oxidation, pyruvate oxidation, amino acid metabolism, and the biosynthesis of acetylcholine, steroid hormones, and melatonin. The trilithium salt form provides excellent aqueous solubility and stability compared to the free acid or sodium salt forms. Each lot is assayed by HPLC (>=95% pure) with controlled levels of oxidized CoA (CoA disulfide) and other degradation products. Lyophilized powder format ensures long-term stability. |
| Intended Use | Cofactor for enzymatic assays involving acetyl-CoA or other acyl-CoA-dependent enzymes (citrate synthase, acetyl-CoA carboxylase, carnitine acetyltransferase, choline acetyltransferase); substrate for CoA biosynthesis pathway studies; component for coupled enzyme assay systems; standard for chromatographic and mass spectrometric CoA quantification. |
| Principle / Technology | Coenzyme A (C21H36N7O16P3S, MW 767.53 as free acid) functions as a carrier of acyl groups via the formation of a high-energy thioester bond at the terminal sulfhydryl group of the beta-mercaptoethylamine moiety. The trilithium salt form (C21H33Li3N7O16P3S) dissociates readily in aqueous solution to yield the active cofactor. |
| Detection Method | Reconstitute in ultrapure water or appropriate buffer to desired concentration; typical working concentrations: 0.1-1 mM for enzymatic assays; prepare fresh solutions and keep on ice (CoA is susceptible to oxidation at the free sulfhydryl group); determine actual concentration spectrophotometrically at 260 nm (epsilon = 16.4 mM^-1 cm^-1) or by DTNB (Ellman's) assay for free -SH groups. |
| Sample Type | Lyophilized powder; reconstitute in water or buffer before use. Avoid exposure to air, moisture, and light. |
| Performance Range / Specifications | Purity: >=95% by HPLC (C18 reverse phase, UV detection at 260 nm); water content: <5% by Karl Fischer titration; oxidized CoA: <3% by HPLC; lithium content: theoretical 3 Li+ per CoA molecule (approximately 2.7% w/w). |
| Sensitivity / LOD | Minimal detectable CoA by DTNB assay: approximately 1 nmol/mL (0.001 A412 units); HPLC detection limit approximately 10 pmol injected (UV 260 nm). |
| Specificity | Enzymatically active CoA with free sulfhydryl group confirmed by positive DTNB reaction and functional assay with phosphate acetyltransferase and citrate synthase coupled system; CoA disulfide (oxidized CoA) is inactive in acyl-transfer reactions and is controlled to <3%. |
| Reaction Conditions / Protocol | Enzymatic assay conditions: varies by target enzyme; typical assay pH 7.0-8.0; CoA functions optimally at neutral to slightly alkaline pH; free sulfhydryl pKa approximately 9.6, deprotonated form is the active nucleophile for thioester formation. |
| Components / Formulation | Coenzyme A trilithium salt, lyophilized powder, >=95% purity; Certificate of Analysis with HPLC chromatogram, water content, and specific rotation. |
| Storage Conditions | Store at -20 C, desiccated, protected from light and moisture; allow vial to equilibrate to RT before opening to prevent moisture condensation. |
| Shelf Life | 36 months from date of manufacture at -20 C; 12 months at 2-8 C desiccated; once reconstituted, solution stable at -20 C for 1 month (aliquot to avoid repeated freeze-thaw). |
| Package Specifications | 25 mg, 50 mg, 100 mg, 250 mg, 500 mg, 1 g. |
| Product Form | White to off-white lyophilized powder; soluble in water at >=50 mg/mL (clear, colorless solution). |
| Quality Control | Each lot tested by: HPLC purity (C18 column, phosphate-acetonitrile gradient, 260 nm, >=95% total CoA); water content by Karl Fischer (<5%); free sulfhydryl by DTNB assay; oxidized CoA by HPLC (<3%); enzymatic activity in coupled citrate synthase/malate dehydrogenase assay; residual solvents by GC (ethanol <0.5%); lithium content by ion chromatography or ICP-OES. |
| Key Features | HPLC >=95% pure; trilithium salt for enhanced solubility; low oxidized CoA (<3%); lyophilized for stability; Certificate of Analysis provided; assayed for enzymatic activity. |
| Purity | >=95% by HPLC; oxidized CoA <3%; water <5%; residual ethanol <0.5%. |
| Concentration | >=95% pure; reconstitution to 10-100 mM typical for stock solutions. |
| Activity / Unit Definition | Enzymatically active: reduction of NAD+ in coupled citrate synthase/malate dehydrogenase assay proportional to CoA concentration; free -SH content >=85% of theoretical by DTNB assay. |
| Molecular Weight | 767.53 g/mol (free acid); trilithium salt approximately 788.43 g/mol; exact molecular formula C21H33Li3N7O16P3S. |
| Source / Origin | Fermentation-derived from microbial source; purified by ion exchange chromatography and preparative HPLC; lithium salt formation by controlled titration with lithium hydroxide. |
| pH Range / Optimal pH | 5% aqueous solution: pH 3.0-5.0; optimal stability in acidic to neutral solutions; alkaline pH accelerates sulfhydryl oxidation. |
| Shipping Conditions | Ambient temperature with desiccant; cold pack recommended for summer transit. |
| Expiration Date / Stability | 36 months at -20 C desiccated; 12 months at 2-8 C; reconstituted solutions at neutral pH stable at -20 C for 1 month (aliquot to avoid freeze-thaw). |
| Regulatory / Compliance | For research use only; not for diagnostic or therapeutic use; not for human or animal drug use. This product is a biochemical reagent for laboratory research. |
| Compatibility | Soluble in water (>50 mg/mL), aqueous buffers (phosphate, Tris, HEPES), and DMSO. Rapidly oxidizes in air to form inactive CoA disulfide; always handle solutions under inert atmosphere (nitrogen or argon) or add 1-5 mM DTT or TCEP as reducing agent. Compatible with Mg2+, Mn2+, and Ca2+ at standard assay concentrations (1-10 mM); precipitation may occur with high concentrations of divalent cations at alkaline pH. |
| Recommended Buffer System | Reconstitute in water or 50 mM potassium phosphate pH 6.5-7.0 for optimal stability; add 1 mM DTT or TCEP for prolonged storage. |
| Application Notes / Precautions | Coenzyme A is hygroscopic and oxidizes readily. After opening the vial, aliquot the remaining solid into single-use portions under dry nitrogen or argon and store at -20 C. Prepare solutions fresh immediately before use; if storage is necessary, de-gas the buffer with nitrogen before solubilization, aliquot promptly, and store at -80 C. Determine actual concentration spectrophotometrically before critical experiments: dilute in water and measure A260; concentration (mM) = A260 / 16.4 x dilution factor. Confirm free -SH content by DTNB assay: mix CoA solution with 100 uM DTNB in 100 mM Tris pH 8.0, measure A412 (epsilon = 14,150 M^-1 cm^-1 for TNB2-). |
| Batch-to-Batch Consistency | HPLC purity >=95% for all lots; oxidized CoA <3%; DTNB-reactive free -SH >=85% of total CoA. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
