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| Product Name | Clonogenic Survival Assay Reagent Kit |
| Catalog No. | CATR-HMM-0048 |
| Description | A complete reagent system for the long-term colony formation assay (clonogenic assay) that determines the reproductive integrity of cells following exposure to ionizing radiation, chemotherapeutic agents, or other cytotoxic treatments. The kit provides fixation and staining reagents optimized for mammalian cell colony visualization and counting. |
| Intended Use | Assessment of long-term proliferative survival and reproductive cell death following radiation therapy research, chemotherapy efficacy testing, and investigation of DNA damage repair mechanisms in cancer biology. |
| Principle / Technology | Single cells are seeded at low density and cultured undisturbed for 1–3 weeks to form colonies; colonies containing ≥50 cells are scored as survivors representing cells that retained unlimited proliferative capacity; fixation with glutaraldehyde preserves colony morphology, and Crystal Violet or Giemsa staining enables visual enumeration. |
| Detection Method | Visual colony counting under stereomicroscope or automated colony counter after colorimetric staining; colonies ≥50 cells (approximately 0.5 mm diameter) scored as survivors |
| Sample Type | Adherent mammalian tumor cell lines (HeLa, MCF-7, HCT116, A549, etc.) and non-transformed fibroblast lines; primary cells with adherent growth characteristics |
| Performance Range / Specifications | Plating efficiency: 50–90% for transformed lines, 5–30% for primary cells; colony formation period: 7–21 days culture; linear dose-response relationship for radiation doses 0–8 Gy |
| Sensitivity / LOD | Detection of single surviving cell capable of forming a colony of ≥50 progeny; plating efficiency assay provides detection limit as low as 0.1% survival fraction |
| Specificity | Only cells capable of sustained proliferation beyond 5–6 cell divisions form countable colonies; growth-arrested but metabolically active cells are not scored as survivors, distinguishing clonogenic survival from short-term viability |
| Reaction Conditions / Protocol | Harvest single-cell suspension, count and seed at appropriate densities (100–10,000 cells/well depending on expected survival); incubate 1–3 weeks without disturbance; fix with glutaraldehyde or methanol; stain with Crystal Violet solution; rinse, dry, and manually count colonies or analyze with imaging software |
| Components / Formulation | Crystal Violet Staining Solution (0.5% in 25% methanol), Fixation Solution (glutaraldehyde 6.0% or methanol), PBS washing buffer; optional colony dissolving solution (1% SDS) for spectrophotometric quantification |
| Storage Conditions | All reagents at room temperature; Crystal Violet solution stable 24 months; glutaraldehyde fixative (if included) stable 12 months at 4°C |
| Shelf Life | 24 months for staining components; 12 months for fixative |
| Package Specifications | Reagents sufficient for fixation and staining of 10–20 × 6-well or 10-cm plates |
| Product Form | Liquid ready-to-use fixation and staining solutions |
| Quality Control | Each lot of stain validated for consistent colony staining intensity across HeLa and MCF-7 cell lines; fixation efficiency confirmed by colony preservation after washing steps |
| Key Features | Gold-standard clonogenicity assay recognized by radiation oncology and cancer pharmacology research communities; measures true reproductive cell survival rather than short-term viability; permanent stained plates provide archival record; compatible with manual and automated colony counting methods |
For research use only, not for clinical use.
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