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| Product Name | Cholesterol Oxidase Enzyme for Total Cholesterol Assay >=11 U/mg 60 kDa Potassium-Ion Stabilized CAS 9028-76-6 |
| Catalog No. | IEC-HMM-0287 |
| Description | Cholesterol oxidase from Alta DiagnoTech oxidizes cholesterol to cholest-4-en-3-one with production of hydrogen peroxide. It is a key enzyme in total cholesterol clinical chemistry reagents. |
| Intended Use | Core enzyme raw material for total cholesterol assay kits. |
| Principle / Technology | Oxidizes cholesterol to cholest-4-en-3-one and H2O2; the peroxide is detected via peroxidase-chromogen coupling. |
| Detection Method | Colorimetric Trinder-type endpoint assay. |
| Sample Type | Serum, plasma. |
| Performance Range / Specifications | Specific activity >=11 U/mg. Molecular weight approximately 60 kDa. Isoelectric point 5.2. |
| Components / Formulation | Cholesterol oxidase lyophilized powder. |
| Storage Conditions | -20°C. |
| Shelf Life | Stable for at least 12 months at -20°C. |
| Package Specifications | KU packaging units. |
| Product Form | Pale yellow amorphous powder. |
| Purity | 99% |
| Activity / Unit Definition | >=11 U/mg |
| Molecular Weight | Approximately 60 kDa |
| Source / Origin | Microbial fermentation. |
| pH Range / Optimal pH | Optimal pH 6.5-7.5; stable between pH 6.0-10.0. |
| Shipping Conditions | Cold chain shipment. |
| Expiration Date / Stability | -20°C stable for one year. |
| Appearance | Pale yellow amorphous powder. |
| CAS Number | 9028-76-6 |
| Isoelectric Point | 5.2 |
| Inhibitors | Not inhibited by sodium azide. |
| Optimal Temperature | 52°C. |
| Thermal Stability | Stable below 60°C. |
| Handling Precaution | Potassium ions help stabilize activity; avoid repeated freeze-thaw. |
| Michaelis Constant | 1×10^-4 M (Cholesterol) |
| Stabilizer | Potassium ions help stabilize enzyme activity. |
For research use only, not for clinical use.
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