Cholesterol Oxidase Enzyme for Total Cholesterol Assay >=11 U/mg 60 kDa Potassium-Ion Stabilized CAS 9028-76-6
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Cholesterol Oxidase Enzyme for Total Cholesterol Assay >=11 U/mg 60 kDa Potassium-Ion Stabilized CAS 9028-76-6

Cat.No: IEC-HMM-0287 Datasheet

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Product Name Cholesterol Oxidase Enzyme for Total Cholesterol Assay >=11 U/mg 60 kDa Potassium-Ion Stabilized CAS 9028-76-6
Catalog No. IEC-HMM-0287
Description Cholesterol oxidase from Alta DiagnoTech oxidizes cholesterol to cholest-4-en-3-one with production of hydrogen peroxide. It is a key enzyme in total cholesterol clinical chemistry reagents.
Intended Use Core enzyme raw material for total cholesterol assay kits.
Principle / Technology Oxidizes cholesterol to cholest-4-en-3-one and H2O2; the peroxide is detected via peroxidase-chromogen coupling.
Detection Method Colorimetric Trinder-type endpoint assay.
Sample Type Serum, plasma.
Performance Range / Specifications Specific activity >=11 U/mg. Molecular weight approximately 60 kDa. Isoelectric point 5.2.
Components / Formulation Cholesterol oxidase lyophilized powder.
Storage Conditions -20°C.
Shelf Life Stable for at least 12 months at -20°C.
Package Specifications KU packaging units.
Product Form Pale yellow amorphous powder.
Purity 99%
Activity / Unit Definition >=11 U/mg
Molecular Weight Approximately 60 kDa
Source / Origin Microbial fermentation.
pH Range / Optimal pH Optimal pH 6.5-7.5; stable between pH 6.0-10.0.
Shipping Conditions Cold chain shipment.
Expiration Date / Stability -20°C stable for one year.
Appearance Pale yellow amorphous powder.
CAS Number 9028-76-6
Isoelectric Point 5.2
Inhibitors Not inhibited by sodium azide.
Optimal Temperature 52°C.
Thermal Stability Stable below 60°C.
Handling Precaution Potassium ions help stabilize activity; avoid repeated freeze-thaw.
Michaelis Constant 1×10^-4 M (Cholesterol)
Stabilizer Potassium ions help stabilize enzyme activity.

For research use only, not for clinical use.

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