| Product Name | Cholesterol Assay Reagent |
| Catalog No. | CMTR-HMM-0021 |
| Description | High-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) are all lipoproteins that function as carrier proteins for cholesterol. The regulation of HDL cholesterol and LDL cholesterol plays a central role in the development of various diseases. |
| Application | This product is used for the quantitative determination of total cholesterol, free cholesterol, and cholesterol esters in mammalian samples. |
| Principle | Cholesterol in blood primarily exists in the form of cholesterol esters. During the assay, cholesterol esterase hydrolyzes cholesterol esters into cholesterol. Cholesterol is then oxidized by cholesterol oxidase to produce hydrogen peroxide. Hydrogen peroxide is subsequently processed by enzymes and reacts with a probe to generate a red color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). |
| Synonyms | High density lipoprotein; LDL; Low density lipoprotein; Very low density lipoprotein; VLDL |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h |
| Sample Type | Other biological fluids, Serum, Plasma, Tissue lysate |
| Procedure | 1. Measure cholesterol using the entire sample; or separate HDL and LDL/VLDL by mixing the sample with precipitation buffer, incubating for 10 minutes, centrifuging at 2,000 g for 10 minutes, retaining the supernatant of the HDL fraction, and repeating centrifugation and resuspending the LDL/VLDL fraction precipitate. 2. During analysis, add the sample and standard to the wells. 3. Add the total cholesterol reaction mixture (containing esterase) or free cholesterol reaction mixture (without esterase), and incubate at 37°C for 60 minutes. 4. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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