| Product Name | Glucose Uptake Assay Reagent |
| Catalog No. | CMTR-HMM-0022 |
| Description | Glucose is a ubiquitous energy source in most organisms, from bacteria to humans. Carbohydrate breakdown produces monosaccharides and disaccharides, most of which are glucose. Through glycolysis and the tricarboxylic acid (TCA) cycle, glucose is oxidized, ultimately forming carbon dioxide and water, and generating the universal energy molecule ATP. Glucose is the primary energy source for the brain and a key component of protein synthesis and lipid metabolism. Therefore, measuring blood glucose levels is a critical diagnostic parameter for many metabolic disorders. |
| Application | This product can detect glucose uptake as low as 10 pmol/well in various cell types. |
| Principle | 2-DG is absorbed by glucose transporters and metabolized into 2-DG-6-phosphate (2-DG6P). 2-DG6P cannot be further metabolized and therefore accumulates within the cell. The accumulation of 2-DG6P is directly proportional to the cell's uptake of 2-DG (or glucose). In this assay, 2-DG6P is oxidized to generate NADPH, whose levels can be measured via an enzymatic cyclic amplification reaction. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Detection Time | 3 h |
| Sample Type | Suspension cells, Adherent cells |
| Detection Limit | 0.01 nmol/well |
| Procedure | 1. Prepare cells using an appropriate glucose starvation/uptake stimulus according to the experimental setup. 2. Add 2-DG to the cells and incubate at 37°C for 20 minutes. 3. Wash the cells with PBS to remove exogenous 2-DG. 4. Lysate the cells with extraction buffer and vortex repeatedly. 5. Freeze/thaw the lysate and heat at 85°C for 40 minutes 6. Cool on ice for 5 minutes 7. Add neutralization buffer, centrifuge, and transfer the supernatant to a new tube 8. Add the supernatant and standards to the wells 9. Add reaction mixture A and incubate at 37°C for 1 hour 10. Add extraction buffer and heat to 90°C for 40 minutes 11. Cool on ice for 5 minutes and add neutralization buffer 12. Add reaction mixture B 13. Read on a microplate reader in kinetic mode every 2–3 minutes at 37°C |
| Storage | -20°C |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2025 Alta DiagenoTech. All rights reserved.
